ExoCoBio Exosome Institute (EEI), ExoCoBio Inc., Seoul, Republic of Korea.
Department of Dermatology, College of Medicine, Dankook University, Cheonan, Republic of Korea.
Int J Dermatol. 2024 Sep;63(9):1212-1220. doi: 10.1111/ijd.17406. Epub 2024 Aug 18.
Androgenetic alopecia (AGA) is a prevalent hair loss disorder with psychological repercussions. Traditional treatments have limitations, leading to the exploration of regenerative therapies such as exosomes derived from adipose tissue stem cells (ASC-Exosomes).
First, using human hair follicle (HF) dermal papilla cells (hDPCs) treated with ASC-Exosomes, ALP, VCAN, β-catenin, and LEF-1 levels with RT-PCR and p-GSK3β, GSK3β, β-catenin, ALP, and β-actin levels with western blot analysis were assessed. Hair shaft elongation test and assay for ALP, Ki-67, and β-catenin were done using human HF organ culture. Patients with AGA had ASC-Exosomes treatment and were evaluated for hair counts, photographic assessments, subjective satisfaction, and safety profiles.
ASC-Exosomes impact hDPCs, increasing proliferation and the upregulation of hair growth-related genes, including ALP, VCAN, β-catenin, and LEF-1. The Wnt/β-catenin pathway was activated, indicating their role in promoting hair growth. ASC-Exosomes also promoted hair shaft elongation and ALP activity, suggesting a potential for hair regeneration. Thirty participants with AGA enrolled and treated over 24 weeks. The subjects experienced a significant increase in total hair density, improved global photographic assessments, and reported higher subjective satisfaction without severe adverse reactions.
This research contributes to the growing body of evidence supporting the use of exosomes in hair loss treatment, offering a safe and effective alternative for individuals with AGA.
雄激素性脱发（AGA）是一种常见的脱发疾病，会对心理健康产生影响。传统治疗方法存在局限性，因此需要探索再生疗法，例如脂肪组织干细胞衍生的外泌体（ASC-Exosomes）。
首先，使用经 ASC-Exosomes 处理的人毛囊真皮乳头细胞（hDPCs），通过 RT-PCR 和 Western blot 分析评估碱性磷酸酶（ALP）、VCAN、β-连环蛋白（β-catenin）和淋巴细胞增强因子 1（LEF-1）的水平，以及 p-GSK3β、GSK3β、β-catenin、ALP 和 β-肌动蛋白的水平。用人毛囊器官培养进行毛干伸长试验以及碱性磷酸酶（ALP）、Ki-67 和 β-catenin 的测定。AGA 患者接受 ASC-Exosomes 治疗，并评估头发计数、照片评估、主观满意度和安全性。
ASC-Exosomes 对 hDPCs 具有影响，可增加增殖并上调与头发生长相关的基因，包括 ALP、VCAN、β-catenin 和 LEF-1。Wnt/β-catenin 通路被激活，表明其在促进头发生长中的作用。ASC-Exosomes 还促进毛干伸长和 ALP 活性，提示其具有促进毛发生长的潜力。30 名 AGA 患者入组并接受了 24 周以上的治疗。患者的总发密度显著增加，全球照片评估得到改善，且主观满意度更高，没有严重的不良反应。
本研究为外泌体在脱发治疗中的应用提供了更多证据，为 AGA 患者提供了一种安全有效的替代治疗方法。
