Translational Research Laboratory, Instituto de Medicina Integral Prof. Fernando Figueira (IMIP), Recife, Brazil.
Hospital de Câncer de Pernambuco, Recife, Brazil.
J Surg Oncol. 2024 Sep;130(4):929-943. doi: 10.1002/jso.27749. Epub 2024 Aug 19.
Penile cancer is high in some underdeveloped countries. Signal transducer and activator of transcription 3 (STAT3) and CD44, CD24, and SOX2+ are known to be markers of diagnosis and prognosis in other cancers, but without studies in penile cancer.
A cross-sectional study was conducted at the Hospital de Cancer de Pernambuco from March 2015 to December 2017. We performed SOX2, STAT3, CD24, and CD44 analyses in blood and tumor tissue by flow cytometry.
High levels of CD44CD24, CD44CD24pSTAT3+ and CD44 CD24 in the blood of patients compared to the controls (p < 0.05). Low of SOX2+ T cells in blood of patients compared to controls. High CD44CD24 levels in patients with perineural invasion (PNI), tumor size > 3 cm, and pT2 stage (p < 0.05). High T cell levels in the blood and tumor tissue of patients with tumor ≤3 cm (p < 0.05). Increased SOX2+ T cells in blood of patients with PNI (-) and pT1 stage (p < 0.05). CD44CD24lpSTAT3+ (r = 0.669; p = 0.024) and SOX2+T cells (r = 0.404, p = 0.029) correlation were observed between blood and tumor tissue in penile cancer patients.
CD44, CD24, and SOX2 molecules were markers of advanced disease associated with the worst prognosis in CaPe. However, pSTAT3 and T cells were associated with a more favorable prognosis in this study.
在一些欠发达国家，阴茎癌的发病率较高。信号转导子和转录激活子 3（STAT3）以及 CD44、CD24 和 SOX2+ 已被证实是其他癌症诊断和预后的标志物，但在阴茎癌中尚无相关研究。
本研究为 2015 年 3 月至 2017 年 12 月在巴西伯南布哥癌症医院进行的一项横断面研究。我们通过流式细胞术分析了血液和肿瘤组织中的 SOX2、STAT3、CD24 和 CD44。
与对照组相比，患者血液中 CD44CD24、CD44CD24pSTAT3+ 和 CD44 CD24 的水平较高（p<0.05）。与对照组相比，患者血液中 SOX2+T 细胞水平较低。在有神经周围侵犯（PNI）、肿瘤大小>3cm 和 pT2 期的患者中，CD44CD24 水平较高（p<0.05）。在肿瘤大小≤3cm 的患者中，患者血液和肿瘤组织中的 T 细胞水平较高（p<0.05）。在无 PNI（-）和 pT1 期的患者中，血液中 SOX2+T 细胞增加（p<0.05）。在阴茎癌患者中，血液和肿瘤组织中 CD44CD24lpSTAT3+（r=0.669;p=0.024）和 SOX2+T 细胞（r=0.404;p=0.029）之间存在相关性。
在 CaPe 中，CD44、CD24 和 SOX2 分子是与疾病进展相关的标志物，与最差的预后相关。然而，在本研究中，pSTAT3 和 T 细胞与更好的预后相关。
