Naziri Z, Hajihasani A, Derakhshandeh A
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
MSc Student in Bacteriology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Vet Res. 2024;25(1):25-32. doi: 10.22099/IJVR.2024.48215.7019.
With the increase in human population, the consumption of livestock products such as sheep meat has also increased. Sheep are the reservoir and shedder of that can be transmitted to humans. Characterization of fecal isolated from sheep in slaughterhouse.
Stool specimens were collected from 30 apparently healthy sheep from different flocks in Shiraz industrial slaughterhouse. The resistance of isolates against 10 antibiotics was determined by disk diffusion method. The presence of three major extended spectrum beta-lactamase (ESBL) genes and five tetracycline resistance genes as well as seven virulence genes were investigated by polymerase chain reaction (PCR) technique. Using the microtiter plate method, the biofilm formation ability of isolates was investigated.
The highest frequency of resistance was to amoxicillin (100%) followed by tetracycline (25%). All isolates were susceptible to gentamicin and nitrofurantoin, and only one isolate was resistant to the tested third-generation cephalosporins. Multidrug resistance phenotype was observed in 16.7% of the isolates. (25%) was the most prevalent ESBL gene and (62.5%) was the most prevalent tetracycline resistance gene in the isolates. , , , and genes were present in all isolates, and the prevalence of and genes was 95.8% and 83.3%, respectively. In total, 62.5% of the isolates were biofilm producers.
According to the concept of One Health, the presence of virulent antibiotic-resistant biofilm producing strains of in sheep is a risk to public health.
随着人口增长，羊肉等畜产品的消费量也在增加。绵羊是可传播给人类的[病原体名称未给出]的储存宿主和传播者。屠宰场绵羊粪便中[病原体名称未给出]的特征分析。
从设拉子工业屠宰场不同羊群的30只外观健康的绵羊中采集粪便样本。采用纸片扩散法测定[病原体名称未给出]分离株对10种抗生素的耐药性。通过聚合酶链反应（PCR）技术检测三种主要的超广谱β-内酰胺酶（ESBL）基因、五种四环素耐药基因以及七种毒力基因的存在情况。采用微量滴定板法研究[病原体名称未给出]分离株的生物膜形成能力。
耐药率最高的是阿莫西林（100%），其次是四环素（25%）。所有[病原体名称未给出]分离株对庆大霉素和呋喃妥因敏感，仅一株分离株对测试的第三代头孢菌素耐药。16.7%的分离株表现出多重耐药表型。[ESBL基因名称未给出]（25%）是分离株中最常见的ESBL基因，[四环素耐药基因名称未给出]（62.5%）是最常见的四环素耐药基因。[毒力基因名称未给出]、[毒力基因名称未给出]、[毒力基因名称未给出]和[毒力基因名称未给出]基因在所有分离株中均存在，[毒力基因名称未给出]和[毒力基因名称未给出]基因的流行率分别为95.8%和83.3%。总体而言，62.5%的分离株是生物膜产生菌。
根据“同一健康”理念，绵羊中存在产毒力、耐抗生素且形成生物膜的[病原体名称未给出]菌株对公众健康构成风险。
