Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, College of Life Sciences and Technology, Tarim University, Alar, 86-843300, China.
Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control of Xinjiang Production and Construction Corps, College of Animal Sciences and Technology, Tarim University, Alar, 86-843300, China.
Folia Microbiol (Praha). 2024 Jun;69(3):567-578. doi: 10.1007/s12223-023-01082-8. Epub 2023 Aug 4.
Despite numerous studies on Escherichia coli (E. coli) from sheep, there have been few reports on the characterization of E. coli isolates from various organs of individual sheep until now. The present study conducted molecular typing, antibiotics resistance, biofilm formation, and virulence genes on E. coli isolated from 57 freshly slaughtered apparently healthy sheep carcasses, gallbladders, fecal samples, and mesenteric lymph nodes (MLNs). The results demonstrated that the detection rate of R1 LPS core type in E. coli isolated from fecal samples (70.83%) was higher than that from other organs, but the detection rate of antibiotic resistance genes was lower (P < 0.05). The predominant phylogenetic group of E. coli isolated from the carcasses was group B1 (93.33%), and the detection rate of multidrug-resistance phenotype (80%) and the resistance rate of E. coli was higher than that from other organs (P < 0.05). Interestingly, the intensity of biofilm formation of E. coli isolated from MLNs was higher than that from other organs (P < 0.05). However, except for ibeB, the detection rates of virulence genes did not differ in E.coli isolated from different organs. In conclusion, differences were noted in these parameters of E. coli isolated from different organs of individual sheep. Therefore, the data may contain considerable mistakes concerning the actual situation in the host if we only analyze the data of E. coli isolated from feces or carcasses.
尽管已经有许多关于绵羊源大肠杆菌（E. coli）的研究，但直到现在，对于个体绵羊不同器官来源的大肠杆菌分离株的特征描述仍鲜有报道。本研究对 57 头新鲜屠宰的、外观健康绵羊的粪便、胆囊、粪便样本和肠系膜淋巴结（MLNs）中的大肠杆菌进行了分子分型、抗生素耐药性、生物膜形成和毒力基因分析。结果表明，粪便样本中分离的大肠杆菌 R1 LPS 核心型的检出率（70.83%）高于其他器官，但抗生素耐药基因的检出率较低（P<0.05）。从胴体中分离的大肠杆菌的主要进化群是 B1 群（93.33%），多药耐药表型的检出率（80%）和大肠杆菌的耐药率均高于其他器官（P<0.05）。有趣的是，从 MLNs 中分离的大肠杆菌的生物膜形成强度高于其他器官（P<0.05）。然而，除了 ibeB 基因外，不同器官来源的大肠杆菌的毒力基因检出率没有差异。总之，个体绵羊不同器官来源的大肠杆菌在这些参数上存在差异。因此，如果我们仅分析粪便或胴体中分离的大肠杆菌的数据，可能会包含关于宿主实际情况的大量错误。
