Shabana I I, Al-Enazi A T
Faculty of Veterinary Medicine, Department of Bacteriology, Immunology and Mycology, Suez Canal University, Egypt.
Biology Department, Faculty of Science, Taibah University, Al-madinah Al-munawarah, Saudi Arabia.
Saudi J Biol Sci. 2020 Mar;27(3):788-796. doi: 10.1016/j.sjbs.2020.01.009. Epub 2020 Jan 16.
is zoonotic bacteria and the emergence of antimicrobial-resistant strains becomes a critical issue in both human and animal health globally. This study was therefore aimed to investigate the plasmid-mediated resistance in strains isolated from healthy and diarrheic sheep and goats. A total of 234 fecal samples were obtained from 157 sheep (99 healthy and 58 diarrheic) and 77 goats (32 healthy and 45 diarrheic) for the isolation and identification of . Plasmid DNA was extracted using the alkaline lysis method. Phenotypic antibiotic susceptibility profiles were determined against the three classes of antimicrobials, which resistance is mediated by plasmids (Cephalosporins, Fluoroquinolone, and Aminoglycosides) using the disc-diffusion method. The frequency of plasmid-mediated resistance genes was investigated by PCR. A total of 159 harbored plasmids. The isolates antibiogram showed different patterns of resistance in both healthy and diarrheic animals. A total of (82; 51.5%) strains were multidrug-resistant. gene was detected in all Aminoglycoside-resistant , and the ESBL-producing possessed different genes. Similarly, fluoroquinolone-resistant possessed different genes. On the analysis of the gene sequence of fluoroquinolone-resistant , multiple point mutations were revealed. In conclusion, a high prevalence of with high resistance patterns to antimicrobials was revealed in the current study, in addition to a wide distribution of their resistance determinants. These findings highlight the importance of sheep and goats as reservoirs for the dissemination of MDR and resistance gene horizontal transfer.
是一种人畜共患病细菌，抗菌耐药菌株的出现已成为全球人类和动物健康领域的一个关键问题。因此，本研究旨在调查从健康和腹泻绵羊及山羊中分离出的菌株中的质粒介导耐药性。共从157只绵羊（99只健康和58只腹泻）和77只山羊（32只健康和45只腹泻）中获取了234份粪便样本，用于分离和鉴定。采用碱裂解法提取质粒DNA。使用纸片扩散法针对三类由质粒介导耐药性的抗菌药物（头孢菌素、氟喹诺酮和氨基糖苷类）测定表型抗生素敏感性谱。通过PCR研究质粒介导耐药基因的频率。共有159株携带质粒。分离株的抗菌谱显示健康和腹泻动物中存在不同的耐药模式。共有82株（51.5%）菌株对多种药物耐药。在所有对氨基糖苷类耐药的菌株中均检测到基因，产ESBL的菌株具有不同的基因。同样，对氟喹诺酮耐药的菌株具有不同的基因。在分析对氟喹诺酮耐药菌株的基因序列时，发现了多个点突变。总之，本研究揭示了对抗菌药物具有高耐药模式的菌株的高流行率，以及它们耐药决定因素的广泛分布。这些发现突出了绵羊和山羊作为耐多药菌株传播和耐药基因水平转移宿主的重要性。
