Hosseini Mehri, Akhavan Sepahi Abbas, Amini Kumarss, Bikhof Torbati Maryam, Mousavi Mohsen
Department of Microbiology , North Tehran Branch , Islamic Azad University , Tehran , Iran.
Department of Microbiology , Faculty of Biological Sciences , Islamic Azad University , Tehran , Iran.
Int J Mol Cell Med. 2024;13(1):105-119. doi: 10.22088/IJMCM.BUMS.13.1.105.
Colorectal cancer is one of the most serious malignancies affecting humans. In this study, bioactive chemicals extracted from soil were analyzed for their anti-colorectal-cancer and antibacterial properties. A total of 100 soil samples were collected from Kerman-Iran, incubated in SCA media and the antimicrobial properties were tested using the cross-streak method. Three strains were cultured in ISP4 medium to obtain secondary bioactive compounds. After studying the effects of the bioactive compounds on the HT29 and human foreskin fibroblast (HFF) cell lines, the expression of the and genes was analyzed by real-time PCR and flow cytometry to detect the presence of apoptosis.The isolates show high degree of identification with and due to 16SrDNA sequence homology. Compared to HT-29 cells, extracts had lower cytotoxicity against normal cells (SI=5.88), followed by HFF (SI=4.14). The cell lines demonstrated a dose-dependent significant increase in DNA fragmentation, an increase in the proportion of cells in sub-G1 phase and caused G2/M cell cycle arrest in HT-29 and HFF cells.The bacterial extracts obtained displayed strong antibacterial properties and inhibited the proliferation of HT-29 and HFF cell lines. The treated cells exhibited morphological changes caused by the activation of caspase and proteins. This confirms that -induced apoptosis is mediated by the activation of . Anti-apoptotic gene expression was downregulated by treatment with the extracts. Further studies are needed to understand the antimicrobial properties of .
结直肠癌是影响人类的最严重恶性肿瘤之一。在本研究中，对从土壤中提取的生物活性化学物质的抗结直肠癌和抗菌特性进行了分析。从伊朗克尔曼采集了总共100份土壤样本，在SCA培养基中培养，并使用交叉划线法测试抗菌特性。在ISP4培养基中培养三株菌株以获得次生生物活性化合物。在研究生物活性化合物对HT29和人包皮成纤维细胞（HFF）细胞系的影响后，通过实时PCR和流式细胞术分析基因的表达以检测凋亡的存在。由于16SrDNA序列同源性，分离物与和具有高度的鉴定性。与HT-29细胞相比，提取物对正常细胞的细胞毒性较低（SI = 5.88），其次是HFF（SI = 4.14）。细胞系显示DNA片段化呈剂量依赖性显著增加，亚G1期细胞比例增加，并导致HT-29和HFF细胞的G2/M细胞周期停滞。获得的细菌提取物显示出很强的抗菌特性，并抑制了HT-29和HFF细胞系的增殖。处理后的细胞表现出由半胱天冬酶和蛋白激活引起的形态变化。这证实了诱导的凋亡是由的激活介导的。通过提取物处理，抗凋亡基因表达下调。需要进一步研究以了解的抗菌特性。
