Department of Clinical Immunology and Allergy, Charles University in Prague, Faculty of Medicine in Hradec Kralove, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
Department of Obstetrics and Gynecology, Hospital Most, Most, Czech Republic.
J Obstet Gynaecol. 2024 Dec;44(1):2390575. doi: 10.1080/01443615.2024.2390575. Epub 2024 Aug 19.
This study aimed to assess variations in the absolute counts of various leukocyte subsets in the peripheral blood of women with pregnancies affected by preterm prelabour rupture of membranes (PPROM), in relation to the presence of intra-amniotic inflammation (IAI).
The study included fifty-two women with singleton pregnancies experiencing PPROM. Absolute counts of different leukocyte subpopulations, such as granulocytes, monocytes, lymphocytes, T cells and their subsets, B cells and their subsets, and NK cells and their subsets, were measured in maternal peripheral blood samples using multicolour flow cytometry. IAI was identified by elevated concentrations of interleukin 6 (IL-6) in the amniotic fluid, which was collected through transabdominal amniocentesis.
Women with IAI exhibited higher absolute counts of leukocytes ( = 0.003), granulocytes ( = 0.008), and monocytes ( = 0.009). However, the presence of IAI did not significantly affect the absolute counts of lymphocytes or their subpopulations.
The study found that IAI is associated with changes in the absolute counts of leukocytes from the innate immunity compartment in the peripheral blood of women with pregnancies complicated by PPROM. Conversely, it does not significantly alter the counts of cells from the adaptive immune system. The changes observed may reflect the natural, temporal, and localised characteristics of IAI.
本研究旨在评估在早产胎膜早破（PPROM）孕妇的外周血中，各种白细胞亚群的绝对计数存在差异，与羊膜内炎症（IAI）的存在有关。
该研究纳入了 52 名经历 PPROM 的单胎妊娠妇女。使用多色流式细胞术测量了外周血样本中不同白细胞亚群的绝对计数，如粒细胞、单核细胞、淋巴细胞、T 细胞及其亚群、B 细胞及其亚群和 NK 细胞及其亚群。通过经腹羊膜穿刺术采集羊水，以升高的白细胞介素 6（IL-6）浓度来确定 IAI。
患有 IAI 的妇女白细胞（=0.003）、粒细胞（=0.008）和单核细胞（=0.009）的绝对计数较高。然而，IAI 的存在并不显著影响淋巴细胞或其亚群的绝对计数。
本研究发现，IAI 与 PPROM 孕妇外周血中固有免疫区白细胞的绝对计数变化有关。相反，它不会显著改变适应性免疫系统细胞的计数。观察到的变化可能反映了 IAI 的自然、时间和局部特征。
