Tang Ruicong, Xing Keyu, Tu Jia, Wang Xufeng, Xu Liguang, Chen Wei, Cheng Yunhui, Xu Zhou
Hunan Provincial Key Laboratory of Cytochemistry, School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha, 410114, Hunan, China.
State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha, 410004, Hunan, China.
Appl Biochem Biotechnol. 2025 Jan;197(1):482-496. doi: 10.1007/s12010-024-05015-0. Epub 2024 Aug 19.
In this study, we used Fe-MIL-101 nanozyme to convert lactose into lactitol, and it was proved that Fe-MIL-101 nanozyme has lactase-like activity. Due to the potential health effects of nanomaterials, we evaluated the cytotoxicity of Fe-MIL-101 nanozyme. To reduce the potential toxicity of the nanozyme, we applied centrifugation and membrane filtration. When the membrane aperture size was 100 nm, the residual content of Fe-MIL-101 nanozyme was 14.09 μg/mL. The residual content of Fe-MIL-101 nanozyme was reduced by optimizing time, temperature, and Fe-MIL-101 nanozyme-to-substrate ratio. It was showed that the concentration of Fe was 38.47 mg/kg and the concentration of HBDC was 0 mg/kg under optimized conditions (110℃, 2 h of reaction and the ratio of Fe-MIL-101 nanozyme to substrate is 1:20). The result met the national standard of China. Experiments measuring cytotoxicity, oxidative stress, and cell membrane damage revealed that less than 20 μg/mL Fe-MIL-101 nanozyme had no significant cytotoxicity. Our study findings showed that Fe-MIL-101 nanozyme reduced lactose content in milk.
在本研究中，我们使用铁基金属有机框架材料Fe-MIL-101纳米酶将乳糖转化为乳糖醇，并证明Fe-MIL-101纳米酶具有类乳糖酶活性。由于纳米材料对健康可能存在影响，我们评估了Fe-MIL-101纳米酶的细胞毒性。为降低纳米酶的潜在毒性，我们采用了离心和膜过滤方法。当膜孔径大小为100纳米时，Fe-MIL-101纳米酶的残留量为14.09微克/毫升。通过优化时间、温度以及Fe-MIL-101纳米酶与底物的比例，Fe-MIL-101纳米酶的残留量得以降低。结果表明，在优化条件下（110℃、反应2小时且Fe-MIL-101纳米酶与底物的比例为1:20），铁的浓度为38.47毫克/千克，对苯二甲酸的浓度为0毫克/千克。该结果符合中国国家标准。测量细胞毒性、氧化应激和细胞膜损伤的实验表明，浓度低于20微克/毫升的Fe-MIL-101纳米酶没有明显的细胞毒性。我们的研究结果表明，Fe-MIL-101纳米酶降低了牛奶中的乳糖含量。
