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苦参碱通过调控MAPK/ERK信号通路抑制骨肉瘤细胞增殖的研究

The use of matrine to inhibit osteosarcoma cell proliferation via the regulation of the MAPK/ERK signaling pathway.

作者信息

Huang Xincheng, Zeng Jun, Ruan Siyuan, Lei Zhuolin, Zhang Jingyuan, Cao Hong

机构信息

Department of Traumatic Orthopedics, Renmin Hospital, Hubei University of Medicine, Shiyan, China.

Department of Anesthesiology, Shiyan People's Hospital, Shiyan, China.

出版信息

Front Oncol. 2024 Aug 5;14:1338811. doi: 10.3389/fonc.2024.1338811. eCollection 2024.

DOI:10.3389/fonc.2024.1338811
PMID:39161382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11330765/
Abstract

BACKGROUND

Matrine is an alkaloid extracted from Sophorus beans of the legume family, and it has significant effects and a variety of pharmacological activities. Osteosarcoma(OS) is a common malignant bone tumor that is characterized by high incidence and rapid progression. There have been some preliminary studies on the therapeutic effect of matrine on OS, but the specific mechanism remains unclear.

OBJECTIVE

The aim of this study was to investigate the antitumor effect of matrine on HOS cells and the underlying molecular mechanism.

METHODS

The effects of matrine on the proliferation, apoptosis and cell cycle progression of HOS cells were determined by CCK-8 assay, TUNEL assay and flow cytometry . Wound healing and Transwell invasion assays were used to observe the effect of matrine on the migration and invasion of HOS cells. The mechanism underlying the antitumor effect of matrine on HOS cells was investigated by Western blotting.

RESULTS

Matrine significantly inhibited HOS cell proliferation, promoted HOS cell apoptosis, and arrested HOS cells in the G1 phase of the cell cycle. Both wound healing and Transwell invasion assays showed that matrine inhibited HOS cell migration and invasion. Western blotting results showed that matrine inhibited the activation of the MAPK/ERK signaling pathway. We found that matrine also downregulated Bcl-2 expression, which may be related to protein synthesis inhibition.

CONCLUSION

Matrine can inhibit the proliferation of HOS cells, arrest HOS cells in the G1 phase, and promote HOS cell apoptosis through the MAPK/ERK signaling pathway.

摘要

背景

苦参碱是从豆科植物苦参的种子中提取的一种生物碱，具有显著的作用和多种药理活性。骨肉瘤（OS）是一种常见的恶性骨肿瘤，具有高发病率和快速进展的特点。关于苦参碱对骨肉瘤的治疗作用已有一些初步研究，但具体机制仍不清楚。

目的

本研究旨在探讨苦参碱对骨肉瘤HOS细胞的抗肿瘤作用及其潜在的分子机制。

方法

采用CCK-8法、TUNEL法和流式细胞术检测苦参碱对HOS细胞增殖、凋亡和细胞周期进程的影响。采用划痕愈合实验和Transwell侵袭实验观察苦参碱对HOS细胞迁移和侵袭的影响。通过蛋白质免疫印迹法研究苦参碱对HOS细胞抗肿瘤作用的机制。

结果

苦参碱显著抑制HOS细胞增殖，促进HOS细胞凋亡，并使HOS细胞停滞于细胞周期的G1期。划痕愈合实验和Transwell侵袭实验均显示苦参碱抑制HOS细胞迁移和侵袭。蛋白质免疫印迹结果显示苦参碱抑制MAPK/ERK信号通路的激活。我们发现苦参碱还下调Bcl-2表达，这可能与蛋白质合成抑制有关。

结论

苦参碱可通过MAPK/ERK信号通路抑制HOS细胞增殖，使HOS细胞停滞于G1期，并促进HOS细胞凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/e5659926cab6/fonc-14-1338811-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/d14c719dca0d/fonc-14-1338811-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/9bf85b559a14/fonc-14-1338811-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/37f184784dc6/fonc-14-1338811-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/9495b9971471/fonc-14-1338811-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/e5659926cab6/fonc-14-1338811-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/d14c719dca0d/fonc-14-1338811-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/9bf85b559a14/fonc-14-1338811-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/37f184784dc6/fonc-14-1338811-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/9495b9971471/fonc-14-1338811-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ed1/11330765/e5659926cab6/fonc-14-1338811-g005.jpg

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