Clarke D J, Calder M R, Carr R J, Blake-Coleman B C, Moody S C, Collinge T A
Biosensors. 1985;1(3):213-320. doi: 10.1016/0265-928x(85)85001-x.
Presently, few of the reported (bio)chemical sensor devices have found application in fermentation monitoring and control. Although many devices with desirable selectivities have been reported, few have demonstrated reliability sufficient to encourage significant and widespread application. Chemical sensors (ion-selective electrodes, amperometric detectors, piezoelectric, field-effect transistors, semiconductor, Optrode and optoelectronic sensors), biosensors (based on potentiometric, amperometric, field-effect transistor and conductiometric detectors) and physical detection methods are reviewed with the aim of highlighting the problems of their application in this area. Physical detection principles appear to show promise as reliable and direct monitoring principles. However, even the more reliable discrete (bio)chemical sensor devices require the development of on-line flow sampling and autocalibration methods to demonstrate the necessary reliability. Biosensor devices appear most problematical and it is concluded that continued development of more direct biosensing principles is likely to prove most fruitful.
目前，已报道的（生物）化学传感器设备中，很少有应用于发酵监测与控制的。尽管已经报道了许多具有理想选择性的设备，但很少有能证明其可靠性足以鼓励大规模广泛应用的。本文对化学传感器（离子选择性电极、安培检测器、压电、场效应晶体管、半导体、Optrode和光电传感器）、生物传感器（基于电位、安培、场效应晶体管和电导检测器）以及物理检测方法进行了综述，旨在突出它们在该领域应用中存在的问题。物理检测原理似乎有望成为可靠且直接的监测原理。然而，即使是更可靠的离散（生物）化学传感器设备，也需要开发在线流动采样和自动校准方法，以证明其必要的可靠性。生物传感器设备似乎问题最多，得出的结论是，持续开发更直接的生物传感原理可能会被证明是最有成效的。
