Reshetilov A N
Prikl Biokhim Mikrobiol. 1996 Jan-Feb;32(1):78-93.
Various types of potentiometric and amperometric biosensors are characterized: microbial sensors with Gluconobacter oxydans cells with potentiometric (pH-sensitive field-effect transistor) and amperometric (Clark-type) electrodes for determining glucose; a potentiometric enzymatic electrode with butyrylcholinesterase, which is used in the biosensor designed to detect pesticides; immunosensors with pH-sensitive field-effect transistors which detect the herbicide 2, 4-D; a biosensor for human immunoglobulin G; biosensors with anaerobic bacteria Clostridium thermocellum; chemical and enzymatic sensors containing a photosensitive membrane for determining ammonium ions and urea; and amperometric microbial sensors prepared with Pseudomonas cells for determining naphthalene, biphenyl, and polychlorinated benzoates. Practical applications of the developed models of biosensors to medicine, biotechnology, and environmental monitoring are discussed.
具有氧化葡萄糖杆菌细胞的微生物传感器，带有用于测定葡萄糖的电位型（pH敏感场效应晶体管）和电流型（克拉克型）电极；带有丁酰胆碱酯酶的电位型酶电极，用于检测农药的生物传感器；带有pH敏感场效应晶体管的免疫传感器，用于检测除草剂2,4 - D；用于人免疫球蛋白G的生物传感器；带有厌氧细菌热纤梭菌的生物传感器；含有用于测定铵离子和尿素的光敏膜的化学和酶传感器；以及用假单胞菌细胞制备用于测定萘、联苯和多氯苯甲酸酯的电流型微生物传感器。讨论了所开发的生物传感器模型在医学、生物技术和环境监测中的实际应用。
