Syraji Yonas, Kebebew Mulugeta, Techane Yohannis, Albene Dawit
Department of Biology College of Natural Sciences Arba Minch University, Arba Minch, Ethiopia.
Int J Microbiol. 2024 Aug 12;2024:1981990. doi: 10.1155/2024/1981990. eCollection 2024.
Medicinal plants have a long history of treating diseases in animals and humans in Ethiopia. Nevertheless, not enough research has been done on the antibacterial properties and possible bioactive components of the majority of medicinal plants. Therefore, this study was concerned with the evaluation of the percentage yield, phytochemical, antimicrobial, antifungal, MIC, antiradical activities, phenolic content, and X-ray fluorescence spectroscopy (XRF) analysis of plant extracts. The mean values of antimicrobial, antifungal, MIC, antiradical, phenolic content, and XRF analysis were reported as mean ± standard deviation. The solvent methanol showed a higher degree of yield in leaf and root extract which was 8.45 (22.27%) and 3.12 g (15.58%), respectively, while distilled water extract of leaf and root showed less degree of yield which was 0.22 g (1.10%) and 0.42 g (2.1%), respectively. Qualitative phytochemical analyses of the plant parts have revealed the presence of various components of metabolites like alkaloids, flavonoids, phenol, saponins, tannins, steroids, steroids, terpenoids, triterpenoids, glycosides, anthraquinones, diterpenes, phytosterols, and phlobatannals. gel extracts had shown significant antibacterial and antifungal activity against the test bacterial and fungus, respectively. Moreover, the methanolic gel extracts of demonstrated notable antiradical activity than the leaf and the root. The highest value of phenolic content was obtained in gel, leaf, and root extract which was 61.9 ± 0.5 mg/g, 53.6 ± 0.3 mg/g, and 51.6 ± 0.6 mg/g, respectively. In this study, twelve elements in the plant parts of were determined using XRF spectroscopy. Overall, this research contributes to the understanding of the pharmacological potential of and highlights the importance of further research into its medicinal properties. The results provide valuable insights into the use of medicinal plants to treat diseases and support the development of natural therapeutics.
在埃塞俄比亚，药用植物在治疗动物和人类疾病方面有着悠久的历史。然而，对于大多数药用植物的抗菌特性和可能的生物活性成分，尚未进行足够的研究。因此，本研究关注植物提取物的百分产率、植物化学、抗菌、抗真菌、最低抑菌浓度（MIC）、抗自由基活性、酚类含量以及X射线荧光光谱（XRF）分析。抗菌、抗真菌、MIC、抗自由基、酚类含量和XRF分析的平均值报告为均值±标准差。溶剂甲醇在叶提取物和根提取物中的产率较高，分别为8.45克（22.27%）和3.12克（15.58%），而叶和根的蒸馏水提取物产率较低，分别为0.22克（1.10%）和0.42克（2.1%）。对植物各部分进行的定性植物化学分析表明，存在生物碱、黄酮类、酚类、皂苷、单宁、甾体、甾体、萜类、三萜类、糖苷、蒽醌、二萜类、植物甾醇和鞣花单宁等多种代谢物成分。凝胶提取物分别对受试细菌和真菌显示出显著的抗菌和抗真菌活性。此外，[植物名称]的甲醇凝胶提取物比叶和根表现出更显著的抗自由基活性。酚类含量最高值在[植物名称]凝胶、叶和根提取物中分别为61.9±0.5毫克/克、53.6±0.3毫克/克和51.6±0.6毫克/克。在本研究中，使用XRF光谱法测定了[植物名称]植物各部分中的12种元素。总体而言，本研究有助于了解[植物名称]的药理潜力，并突出了进一步研究其药用特性的重要性。研究结果为利用药用植物治疗疾病提供了有价值的见解，并支持天然疗法的开发。
