Mulatu Getachew
Department Veterinary Laboratory Technology, College of Agriculture and Veterinary Sciences, Ambo University, P.O. Box 19, Ambo, Ethiopia.
Adv Pharmacol Pharm Sci. 2020 Nov 17;2020:8840468. doi: 10.1155/2020/8840468. eCollection 2020.
The study aimed to determine the phytochemicals and to assess the antibacterial activities of crude extracts of different parts of against , , , O157 H:7, , and The fresh and healthy leaves, barks, stems, and roots of the plant parts were collected, herbarium, dried, and grounded, and bioactive compounds were extracted by ethanol (99%) and water. Mass of crude extracts was determined by using the Whatman No. 1 filter paper and rotary evaporator. Major secondary metabolites were also screened using phytochemical screening tests. Antibacterial activities (inhibition zones, mm) and minimum inhibition concentrations (MIC) were evaluated using agar-well diffused methods and agar dilution methods, respectively. The antibiotics ciprofloxacin, amoxicillin, penicillin, and tetracycline were used as positive controls at concentrations of 0.1 mg/ml and 0.2 mg/ml, while distilled water was used as the negative control. All the crude extracts were tested triplet (3x) for antibacterial activities against selected bacterial strains with two different concentrations 25 and 50 mg/ml and analyzed to compare the mean ± standard deviation between triplets. The results revealed that ethanol extracts showed high crude mass extracts, antibacterial activities, and major secondary metabolites such as alkaloids, tennis, flavonoids, saponins, steroids, and phlobatannins compared with aqueous extracts. Among antibiotics used, penicillin showed resistance to and O157 H:7. was found to be the most susceptible bacterium to ethanol extracts' leaves, barks, and stems with MIC 3.125 mg/ml, whereas was the least susceptible to all crude extracts. The study provided the traditional and scientific basis of used against some bacterial diseases.
该研究旨在确定植物不同部位粗提物的植物化学成分，并评估其对[细菌名称1]、[细菌名称2]、[细菌名称3]、O157 H:7、[细菌名称4]和[细菌名称5]的抗菌活性。采集了该植物部位新鲜健康的叶、树皮、茎和根，制作标本，干燥并研磨，然后用乙醇（99%）和水提取生物活性化合物。使用Whatman No. 1滤纸和旋转蒸发仪测定粗提物的质量。还通过植物化学筛选试验对主要次生代谢产物进行了筛选。分别使用琼脂孔扩散法和琼脂稀释法评估抗菌活性（抑菌圈，mm）和最低抑菌浓度（MIC）。抗生素环丙沙星、阿莫西林、青霉素和四环素在浓度为0.1mg/ml和0.2mg/ml时用作阳性对照，而蒸馏水用作阴性对照。所有粗提物针对选定的细菌菌株在25mg/ml和50mg/ml两种不同浓度下进行了三次重复抗菌活性测试，并分析比较三次重复之间的平均值±标准差。结果表明，与水提取物相比，乙醇提取物显示出较高的粗提物质量、抗菌活性以及主要次生代谢产物，如生物碱、单宁、黄酮类、皂苷、甾体和鞣花单宁。在所使用的抗生素中，青霉素对[细菌名称1]和O157 H:7表现出耐药性。发现[细菌名称6]对乙醇提取物的叶、树皮和茎最敏感，MIC为3.125mg/ml，而[细菌名称7]对所有粗提物最不敏感。该研究为该植物用于防治某些细菌性疾病提供了传统和科学依据。
