MOE Key Laboratory for Cellular Dynamics, Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Hefei National Laboratory for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China.
Plant J. 2024 Oct;120(1):318-334. doi: 10.1111/tpj.16988. Epub 2024 Aug 20.
Plants synthesize hundreds of small secretory peptides, which are perceived by the receptor-like kinase (RLK) family at the cell surface. Various signaling peptide-RLK pairs ensure plant adaptation to distinct environmental conditions. Here, we report that SERINE RICH ENDOGENOUS PEPTIDE (SCOOP) immune peptides modulate root growth and development by regulating PIN-FORMED (PIN)-regulated polar auxin transport in Arabidopsis. The SCOOP4 and SCOOP12 treatments impaired root gravitropic growth, auxin redistribution in response to gravistimulation, and PIN abundance in the PM. Furthermore, genetic and cell biological analyses revealed that these physiological and cellular effects of SCOOP4 and SCOOP12 peptides are mediated by the receptor MALE DISCOVERER1-INTERACTING RECEPTOR LIKE KINASE2 (MIK2) and the downstream mitogen-activated kinase MPK6. Biochemical evidence indicates that MPK6 directly phosphorylates the cytosolic loop of PIN proteins. Our work established a link between the immune signaling peptide SCOOPs and root growth pathways, providing insights into the molecular mechanisms underlying plant root adaptive growth in the defense response.
植物合成数百种小分泌肽，这些肽在细胞表面被受体样激酶 (RLK) 家族感知。各种信号肽-RLK 对确保植物适应不同的环境条件。在这里，我们报告丝氨酸丰富的内源性肽（SCOOP）免疫肽通过调节拟南芥中的 PIN 形成（PIN）调节的极性生长素运输来调节根的生长和发育。SCOOP4 和 SCOOP12 处理会损害根向重力性生长、对重刺激的生长素再分布以及 PM 中的 PIN 丰度。此外，遗传和细胞生物学分析表明，SCOOP4 和 SCOOP12 肽的这些生理和细胞效应是由受体雄性发现者 1-相互作用受体样激酶 2 (MIK2) 和下游丝裂原激活的蛋白激酶 MPK6 介导的。生化证据表明，MPK6 直接磷酸化 PIN 蛋白的胞质环。我们的工作在免疫信号肽 SCOOPs 和根生长途径之间建立了联系，为植物根在防御反应中适应性生长的分子机制提供了深入了解。
