The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.
Institute of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich, Zurich, Switzerland.
Nat Commun. 2021 Jan 29;12(1):705. doi: 10.1038/s41467-021-20932-y.
Plant genomes encode hundreds of receptor kinases and peptides, but the number of known plant receptor-ligand pairs is limited. We report that the Arabidopsis leucine-rich repeat receptor kinase LRR-RK MALE DISCOVERER 1-INTERACTING RECEPTOR LIKE KINASE 2 (MIK2) is the receptor for the SERINE RICH ENDOGENOUS PEPTIDE (SCOOP) phytocytokines. MIK2 is necessary and sufficient for immune responses triggered by multiple SCOOP peptides, suggesting that MIK2 is the receptor for this divergent family of peptides. Accordingly, the SCOOP12 peptide directly binds MIK2 and triggers complex formation between MIK2 and the BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) co-receptor. MIK2 is required for resistance to the important root pathogen Fusarium oxysporum. Notably, we reveal that Fusarium proteomes encode SCOOP-like sequences, and corresponding synthetic peptides induce MIK2-dependent immune responses. These results suggest that MIK2 may recognise Fusarium-derived SCOOP-like sequences to induce immunity against Fusarium. The definition of SCOOPs as MIK2 ligands will help to unravel the multiple roles played by MIK2 during plant growth, development and stress responses.
植物基因组编码了数百种受体激酶和肽，但已知的植物受体-配体对的数量是有限的。我们报告说，拟南芥富含亮氨酸重复受体激酶 LRR-RK MALE DISCOVERER 1-INTERACTING RECEPTOR LIKE KINASE 2 (MIK2) 是 SERINE RICH ENDOGENOUS PEPTIDE (SCOOP) 植物细胞因子的受体。MIK2 是多种 SCOOP 肽触发免疫反应所必需和充分的，这表明 MIK2 是这个不同家族肽的受体。因此，SCOOP12 肽直接结合 MIK2 并触发 MIK2 与 BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) 共受体之间的复合物形成。MIK2 是对重要的根病原体尖孢镰刀菌抗性所必需的。值得注意的是，我们揭示了尖孢镰刀菌蛋白质组编码 SCOOP 样序列，并且相应的合成肽诱导 MIK2 依赖性免疫反应。这些结果表明，MIK2 可能识别尖孢镰刀菌衍生的 SCOOP 样序列，以诱导对尖孢镰刀菌的免疫。将 SCOOP 定义为 MIK2 的配体将有助于揭示 MIK2 在植物生长、发育和应激反应中的多种作用。
