Lloyd R S, Hanawalt P C
Proc Natl Acad Sci U S A. 1981 May;78(5):2796-800. doi: 10.1073/pnas.78.5.2796.
The denV gene of bacteriophage T4 has been cloned into Escherichia coli K-12 by inserting appropriate fragments of cytosine-containing T4 DNA into the Sal I site of the plasmid pBR322. The denV gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in DNA by UV. In uvrA recA mutants, deficient in an early step in excision repair, the cloned DNA results in enhanced UV resistance that is more pronounced in stationary- than in exponential-phase cultures. The expression of the cloned DNA also results in the enhanced survival of UV-irradiated phage lambda or of a denV mutant of phage T4 and in removal of dimers from the DNA of UV-irradiated cells.
通过将含胞嘧啶的T4 DNA的适当片段插入质粒pBR322的Sal I位点，噬菌体T4的denV基因已被克隆到大肠杆菌K-12中。denV基因编码一种酶，该酶启动紫外线在DNA中产生的嘧啶二聚体的切除修复。在切除修复早期步骤存在缺陷的uvrA recA突变体中，克隆的DNA导致紫外线抗性增强，这在稳定期培养物中比在指数期培养物中更明显。克隆DNA的表达还导致紫外线照射的噬菌体λ或噬菌体T4的denV突变体的存活率提高，并导致从紫外线照射细胞的DNA中去除二聚体。
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