State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Street, Changchun, 130022, PR China; University of Science and Technology of China, Hefei, 230026, PR China; Department of Chemistry, College of Natural Sciences, Arbaminch University, P.O. Box 21, Arbaminch, Ethiopia.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Street, Changchun, 130022, PR China; Department of Pharmacy, College of Medicine and Health Science, Ambo University, P.O. Box 19, Ambo, Ethiopia.
Talanta. 2022 Oct 1;248:123611. doi: 10.1016/j.talanta.2022.123611. Epub 2022 May 29.
Since the content levels of biomarkers at the early stage of many diseases are generally lower than the detection threshold concentration, achieving ultrasensitive and accurate detection of these biomarkers is still one of the major goals in bio-analysis. To achieve ultrasensitive and reliable bioassay, it requires developing highly sensitive biosensors. Among all kinds of biosensors, electrogenerated chemiluminescence (ECL) based biosensors have attracted enormous attention due to their excellent properties. In order to improve the performance of ECL biosensors, gold nanoparticles (Au NPs) have been widely utilized as signal amplification tags. The introduction of Au NPs could dramatically enhance the performance of the constructed ECL biosensors via diverse ways such as electrode modification material, efficient energy acceptor in ECL resonant energy transfer (ECL-RET), reaction catalyst, surface plasmon resonance (SPR) enhancer, and as nanocarrier. Herein, we summarize recent developments and progress of ECL biosensors based on Au NPs signal amplification strategies. We will cover ECL applications of Au NPs as a signal amplification tag in the detection of proteins, metal ions, nucleic acids, small molecules, living cells, exosomes, and cell imaging. Finally, brief summary and future outlooks of this field will be presented.
由于许多疾病早期的生物标志物含量通常低于检测阈值浓度，因此实现对这些生物标志物的超灵敏和准确检测仍然是生物分析的主要目标之一。为了实现超灵敏和可靠的生物分析，需要开发高灵敏度的生物传感器。在各种生物传感器中，基于电致化学发光（ECL）的生物传感器因其优异的性能而引起了极大的关注。为了提高 ECL 生物传感器的性能，金纳米粒子（Au NPs）已被广泛用作信号放大标签。通过多种方式，如电极修饰材料、ECL 共振能量转移（ECL-RET）中的高效能量受体、反应催化剂、表面等离子体共振（SPR）增强剂和纳米载体，引入 Au NPs 可以显著增强构建的 ECL 生物传感器的性能。本文总结了基于 Au NPs 信号放大策略的 ECL 生物传感器的最新发展和进展。我们将涵盖 Au NPs 在蛋白质、金属离子、核酸、小分子、活细胞、外泌体和细胞成像检测中的 ECL 应用。最后，将对该领域进行简要总结和展望。
