Center for Advanced Sensor Technology, Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, Baltimore, MD, 21250, USA.
Biotechnol Lett. 2024 Dec;46(6):983-996. doi: 10.1007/s10529-024-03521-z. Epub 2024 Aug 20.
Although online monitoring of dissolved O, pH, and dissolved CO is critical in bioprocesses, nearly all existing technologies require some level of direct contact with the cell culture environment, posing risks of contamination. This study addresses the need for an accurate, and completely noninvasive technique for simultaneous measurement of these analytes. A "non-contact" technique for simultaneous monitoring of dissolved O pH, and dissolved CO was developed. Instead of direct contact with the culture media, the measurements were made through permeable membranes via either a sampling port in the culture vessel wall or a flow cell. The efficacy of the "non-contact" technique was validated in Escherichia coli (E.coli), Chinese hamster ovary (CHO) culture processes, and dynamic environments created by sparging gases in cell culture medium. The measurements obtained through the developed techniques were comparable to those obtained through control methods. The noninvasive monitoring system can offer accurate, and contamination-minimized monitoring of critical process parameters including dissolved O, pH, and dissolved CO. These advancements will enhance the control and optimization of cell culture processes, promising improved cell culture performance.
虽然在线监测溶解氧、pH 值和溶解二氧化碳对于生物工艺至关重要，但几乎所有现有的技术都需要与细胞培养环境直接接触，从而带来污染风险。本研究旨在满足对这些分析物进行准确、完全非侵入式测量的需求。本文开发了一种“非接触式”技术，可同时监测溶解氧、pH 值和溶解二氧化碳。与直接接触培养基不同，测量是通过培养容器壁上的取样口或流动池穿过透气膜进行的。“非接触式”技术在大肠杆菌（E.coli）、中国仓鼠卵巢（CHO）培养过程以及通过向细胞培养基中鼓入气体产生的动态环境中的功效得到了验证。通过开发的技术获得的测量值与通过对照方法获得的测量值相当。这种非侵入式监测系统可以对包括溶解氧、pH 值和溶解二氧化碳在内的关键过程参数进行准确、污染最小化的监测。这些进展将增强细胞培养过程的控制和优化，有望提高细胞培养性能。
