Department of Applied Chemistry, Kyung Hee University, Yongin, Gyeonggi 17104, Republic of Korea.
Department of Chemistry, Kangwon National University, Chuncheon 24341, Republic of Korea.
Biomacromolecules. 2024 Sep 9;25(9):5959-5967. doi: 10.1021/acs.biomac.4c00623. Epub 2024 Aug 21.
This study investigates the viscosity and liquid-solid transition behavior of biomolecular condensates formed by polyarginine chains (R) of varying lengths and citric acid (CA) derivatives. By condensing R chains of various lengths with CA derivatives, we showed that the shorter R chains attenuate the high aggregation tendency of the longer chains when condensed with CA. A mixture of different R lengths exhibited uniform intracondensate distribution, while its mobility largely depended on the ratio of the longer R chain. Our findings demonstrate a simple method to modulate condensate properties by adjusting the composition of scaffold molecules, shedding light on the role of molecular composition in controlling condensate viscosity and transition dynamics. This research contributes to a deeper understanding of biomolecular condensation processes and offers insights into potential strategies for manipulating condensate properties for various applications, including in the fields of synthetic biology and disease therapeutics in the future.
这项研究调查了由不同长度的聚精氨酸链（R）和柠檬酸（CA）衍生物形成的生物分子凝聚物的粘度和液-固转变行为。通过将不同长度的 R 链与 CA 衍生物凝聚，我们表明，当与 CA 凝聚时，较短的 R 链会减弱较长链的高聚集倾向。不同长度 R 链的混合物表现出均匀的凝聚物内部分布，而其流动性在很大程度上取决于较长 R 链的比例。我们的发现表明，通过调整支架分子的组成来调节凝聚物性质是一种简单的方法，这为分子组成在控制凝聚物粘度和转变动力学中的作用提供了新的认识。这项研究有助于深入了解生物分子凝聚过程，并为未来在合成生物学和疾病治疗等领域操纵凝聚物性质的潜在策略提供了新的思路。
