College of Life Science and Technology, Joint Laboratory of Medical Instrument Innovation, Changchun University of Science and Technology, Changchun, 130000, China.
School of Science, Dalian Maritime University, Dalian, 116026, People's Republic of China.
Mikrochim Acta. 2024 Aug 21;191(9):553. doi: 10.1007/s00604-024-06632-6.
Single-level biomarker detection has the limitation of insufficient accuracy in cancer diagnosis. Therefore, the strategy of developing highly sensitive, multi-channel biosensors for high-throughput ctDNA determination is critical to improve the accuracy of early diagnosis of clinical tumors. Herein, in order to achieve efficient detection of up to ten targets for early diagnosis of ovarian cancer, a DNA-nanoswitch-based multi-channel (DNA-NSMC) biosensor was built based on the multi-module catalytic hairpin assembly-mediated signal amplification (CHA) and toehold-mediated DNA strand displacement (TDSD) reaction. Only two different fluorescence signals were used as outputs, combined with modular segmentation strategy of DNA-nanoswitch-based reaction platform; the multi-channel detection of up to ten targets was successfully achieved for the first time. The experimental results suggest that the proposed biosensor is a promising tool for simultaneously detecting multiple biomarkers for the early diagnosis of ovarian cancer, offering new strategies for the early screening, diagnosis, and treatment not only for ovarian cancer but also for other cancers.
单层次生物标志物检测在癌症诊断中的准确性有限。因此，开发高度敏感、多通道生物传感器以实现 ctDNA 的高通量测定对于提高临床肿瘤早期诊断的准确性至关重要。在此，为了实现多达十种靶标用于卵巢癌早期诊断的高效检测，构建了基于 DNA 纳米开关的多通道（DNA-NSMC）生物传感器，该传感器基于多模块催化发夹组装介导的信号放大（CHA）和链置换反应（TDSD）。该生物传感器仅使用两种不同的荧光信号作为输出，结合 DNA 纳米开关反应平台的模块化分割策略；首次成功实现了多达十种靶标的多通道检测。实验结果表明，该生物传感器有望成为用于卵巢癌早期诊断的多种生物标志物的同时检测的工具，为早期筛查、诊断和治疗不仅卵巢癌，还包括其他癌症提供了新的策略。
