Wu Yu, Wang Chunhua, Wang Chao, Wang Pan, Chang Xiaohan, Han Lin, Zhang Yu
Peking University Third Hospital, Haidian District, Beijing 100191, China.
Institute of Marine Science and Technology, Shandong University, Qingdao 266273, China.
Micromachines (Basel). 2022 Nov 22;13(12):2046. doi: 10.3390/mi13122046.
Ovarian tumors/cancers are threatening women's health worldwide, which demands high-performance detection methods and accurate strategies to effectively detect, diagnose and treat them. Here, we report a nanographene oxide particle-functionalized microfluidic fluorescence biosensor to simultaneously detect four biomarkers, CA125, HE4, CEA and APF, for ovarian tumor/cancer diagnosis. The developed biosensor exhibits good selectivity and a large biomarker detection range with a limit of detection of 0.01 U/mL for CA125 and ~1 pg/mL for HE4, CEA and APF. The current results indicate that (1) the proposed biosensor is a promising tool for the simultaneous detection of multiple biomarkers in ovarian tumors/cancer and (2) CA125 and HE4 are strong indicators, AFP may be helpful, and CEA is a weak biomarker for ovarian tumor/cancer diagnosis. The proposed biosensor would be a potential tool, and an analytical approach for the simultaneous detection of multiple biomarkers will provide a new strategy for the early screening, diagnosis and treatment of ovarian tumors/cancers, as well as other cancers.
卵巢肿瘤/癌症正在全球范围内威胁着女性健康，这就需要高性能的检测方法和准确的策略来有效地检测、诊断和治疗它们。在此，我们报道了一种氧化石墨烯颗粒功能化的微流控荧光生物传感器，用于同时检测四种生物标志物CA125、HE4、CEA和APF，以用于卵巢肿瘤/癌症的诊断。所开发的生物传感器具有良好的选择性和较大的生物标志物检测范围，CA125的检测限为0.01 U/mL，HE4、CEA和APF的检测限约为1 pg/mL。目前的结果表明：（1）所提出的生物传感器是一种用于同时检测卵巢肿瘤/癌症中多种生物标志物的有前景的工具；（2）CA125和HE4是强有力的指标，AFP可能有帮助，而CEA是卵巢肿瘤/癌症诊断的弱生物标志物。所提出的生物传感器将是一种潜在的工具，同时检测多种生物标志物的分析方法将为卵巢肿瘤/癌症以及其他癌症的早期筛查、诊断和治疗提供一种新策略。
