Aburel Oana-Maria, Brăescu Laurențiu, Buriman Darius G, Merce Adrian P, Bînă Anca M, Borza Claudia, Mornoș Cristian, Sturza Adrian, Muntean Danina M
Chair of Pathophysiology, Department III, "Victor Babeş" University of Medicine and Pharmacy of Timișoara, E. Murgu Sq. No. 2, 300041, Timişoara, Romania.
Centre for Translational Research and Systems Medicine, "Victor Babeş" University of Medicine and Pharmacy of Timișoara, E. Murgu Sq. No. 2, 300041, Timişoara, Romania.
Mol Cell Biochem. 2025 Apr;480(4):2413-2421. doi: 10.1007/s11010-024-05092-z. Epub 2024 Aug 21.
Cardiovascular diseases represent the major cause of morbidity mainly due to chronic heart failure. Epicardial (EAT) and perivascular adipose tissues (PVAT) are considered major contributors to the pathogenesis of cardiometabolic pathologies. Monoamine oxidases (MAOs) are mitochondrial enzymes recognized as sources of reactive oxygen species (ROS) in cardiometabolic pathologies. Methylene blue (MB) is one of the oldest protective agents, yet no data are available about its effects on adipose tissue. The present pilot study was aimed at assessing the effects of MB: (i) on MAO expression and (ii) oxidative stress in EAT and PVAT harvested from patients with heart failure subjected to cardiac surgery (n = 25). Adipose tissue samples were incubated with MB (0.1 µM/24 h) and used for the assessment of MAO gene and protein expression (qPCS and immune fluorescence) and ROS production (confocal microscopy and spectrophotometry). The human cardiovascular adipose tissues contain both MAO isoforms, predominantly MAO-A. Incubation with MB reduced MAOs expression and oxidative stress; co-incubation with serotonin, the MAO-A substrate, further augmented ROS generation, an effect partially reversed by MB. In conclusion, MAO-A is the major isoform expressed in EAT and PVAT and contribute to local oxidative stress; both effects can be mitigated by methylene blue.
心血管疾病是发病的主要原因，主要是由于慢性心力衰竭。心外膜脂肪组织（EAT）和血管周围脂肪组织（PVAT）被认为是心脏代谢疾病发病机制的主要促成因素。单胺氧化酶（MAOs）是线粒体酶，在心脏代谢疾病中被认为是活性氧（ROS）的来源。亚甲蓝（MB）是最古老的保护剂之一，但尚无关于其对脂肪组织影响的数据。本初步研究旨在评估MB对：（i）MAO表达和（ii）从接受心脏手术的心力衰竭患者（n = 25）获取的EAT和PVAT中的氧化应激的影响。脂肪组织样本与MB（0.1 µM/24小时）孵育，并用于评估MAO基因和蛋白质表达（定量聚合酶链反应和免疫荧光）以及ROS产生（共聚焦显微镜和分光光度法）。人类心血管脂肪组织含有两种MAO同工型，主要是MAO-A。与MB孵育可降低MAOs表达和氧化应激；与MAO-A底物血清素共同孵育可进一步增强ROS生成，MB可部分逆转这一效应。总之，MAO-A是EAT和PVAT中表达的主要同工型，并导致局部氧化应激；亚甲蓝可减轻这两种效应。
