Cottrelle P, Cool M, Thuriaux P, Price V L, Thiele D, Buhler J M, Fromageot P
Service de Biochimie, C.E.N. Saclay, Gif-sur Yvette, France.
Curr Genet. 1985;9(8):693-7. doi: 10.1007/BF00449823.
Two genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae. We have generated yeast haploid strains containing either TEF1 or TEF2 interrupted by insertion of a large piece of foreign DNA. Cells which contain either one functional copy of the EF-1 alpha genes are viable. In contrast, attempts to isolate a yeast haploid strain with both TEF1 and TEF2 inactivated have failed suggesting that the double gene disruption is a lethal event.
在酿酒酵母中，TEF1和TEF2这两个基因编码蛋白质延伸因子EF-1α。我们构建了酵母单倍体菌株，其中TEF1或TEF2因插入大片段外源DNA而中断。含有一个功能性EF-1α基因拷贝的细胞是可存活的。相比之下，分离TEF1和TEF2均失活的酵母单倍体菌株的尝试失败了，这表明双基因破坏是一个致死事件。
