热处理后通过逆转录聚合酶链反应直接检测受污染牛奶样品中的活菌、霉菌和酵母菌。
Direct detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment.
作者信息
Vaitilingom M, Gendre F, Brignon P
机构信息
Tepral, Beverage Division Research Center of Danone Group, Strasbourg, France.
出版信息
Appl Environ Microbiol. 1998 Mar;64(3):1157-60. doi: 10.1128/AEM.64.3.1157-1160.1998.
Abstract
A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1 alpha)-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other foods and other contaminants.
摘要
开发了一种快速、灵敏且可调节目标污染物的方法，用于检测热处理后的活菌、霉菌和酵母菌。通过使用延伸因子基因（EF-Tu或EF-1α）特异性引物进行逆转录PCR，检测水平为每毫升牛奶10个细胞。该方法的简单性和快速性（4小时）表明，此方法可能易于应用于其他食品和其他污染物的检测。
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