National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan, 572024, China.
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, National Engineering Research Center for Sugarcane, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
Plant J. 2024 Oct;120(1):335-353. doi: 10.1111/tpj.16990. Epub 2024 Aug 21.
12-Oxo-phytodienoic acid reductases (OPRs) perform vital functions in plants. However, few studies have been reported in sugarcane (Saccharum spp.), and it is of great significance to systematically investigates it in sugarcane. Here, 61 ShOPRs, 32 SsOPRs, and 36 SoOPRs were identified from R570 (Saccharum spp. hybrid cultivar R570), AP85-441 (Saccharum spontaneum), and LA-purple (Saccharum officinarum), respectively. These OPRs were phylogenetically classified into four groups, with close genes similar structures. During evolution, OPR gene family was mainly expanded via whole-genome duplications/segmental events and predominantly underwent purifying selection, while sugarcane OPR genes may function differently in response to various stresses. Further, ScOPR2, a tissue-specific OPR, which was localized in cytoplasm and cell membrane and actively response to salicylic acid (SA), methyl jasmonate, and smut pathogen (Sporisorium scitamineum) stresses, was cloned from sugarcane. In addition, both its transient overexpression and stable overexpression enhanced the resistance of transgenic plants to pathogen infection, most probably through activating pathogen-associated molecular pattern/pattern-recognition receptor-triggered immunity, producing reactive oxygen species, and initiating mitogen-activated protein kinase cascade. Subsequently, the transmission of SA and hypersensitive reaction were triggered, which stimulated the transcription of defense-related genes. These findings provide insights into the function of ScOPR2 gene for disease resistance.
12-氧代-植物二烯酸还原酶（OPRs）在植物中发挥着重要的功能。然而，在甘蔗（Saccharum spp.）中报道的研究很少，因此系统地研究甘蔗中的 OPRs 具有重要意义。本研究从 R570（甘蔗属杂交品种 R570）、AP85-441（蔗茅）和 LA-紫色（甘蔗属）中分别鉴定出 61 个 ShOPRs、32 个 SsOPRs 和 36 个 SoOPRs。这些 OPRs 被系统发育分为四个组，具有相似的结构。在进化过程中，OPR 基因家族主要通过全基因组加倍/片段事件进行扩张，主要经历纯化选择，而甘蔗 OPR 基因可能对不同的胁迫做出不同的反应。此外，从甘蔗中克隆出了一个组织特异性的 OPR ScOPR2，它定位于细胞质和细胞膜中，对水杨酸（SA）、茉莉酸甲酯和黑粉病（Sporisorium scitamineum）胁迫有积极的反应。此外，瞬时过表达和稳定过表达 ScOPR2 都增强了转基因植物对病原体感染的抗性，这可能是通过激活病原体相关分子模式/模式识别受体触发的免疫、产生活性氧和启动丝裂原活化蛋白激酶级联反应来实现的。随后，引发了 SA 的传递和过敏反应，从而刺激了防御相关基因的转录。这些发现为 ScOPR2 基因的抗病功能提供了新的见解。
