Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, China.
Biomacromolecules. 2024 Sep 9;25(9):6093-6102. doi: 10.1021/acs.biomac.4c00746. Epub 2024 Aug 21.
Heteropolypeptides bearing two or more functional side chains are promising polymeric materials for various biomedical applications. However, conventional preparation of heteropolypeptides relies on the synthesis and purification of each -carboxyanhydride (NCA) monomer in a separate manner, which substantially increases the time and cost. Herein, we report the facile preparation of heteropolypeptides with up to 86% yield within several hours, which are obtained from a mixture of crude NCA monomers. The combination of -hexane precipitation and biphasic segregation effectively removed >90% impurities from crude NCA mixtures, allowing for the successful polymerization process. Various heteropolypeptides with monomodal distribution and narrow dispersity were efficiently prepared, whose compositions were predetermined by the feeding ratios of amino acids. We believe that this work significantly simplifies the preparation of various heteropolypeptides, boosting the downstream studies of these promising materials.
具有两个或更多功能侧链的杂肽是各种生物医学应用有前途的聚合物材料。然而，杂肽的常规制备依赖于以单独的方式合成和纯化每个 - 羧酸酐 (NCA) 单体，这大大增加了时间和成本。在此，我们报告了在数小时内以高达 86%的产率制备杂肽的简便方法，该方法是从粗 NCA 单体混合物中获得的。正己烷沉淀和两相分离的组合可有效去除粗 NCA 混合物中>90%的杂质，从而实现成功的聚合过程。通过控制氨基酸的进料比，可有效地制备具有单分散性和窄分散性的各种杂肽，其组成是预先确定的。我们相信这项工作极大地简化了各种杂肽的制备，推动了这些有前途的材料的下游研究。
