• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酵母线粒体DNA中cob基因的缺失及其表型效应。

Deletions in the cob gene of yeast mtDNA and their phenotypic effect.

作者信息

Dobres M, Gerbl-Rieger S, Schmelzer C, Mueller M W, Schweyen R J

机构信息

Institute für Genetik und Mikrobiologie, Universität München, Federal Republic of Germany.

出版信息

Curr Genet. 1985;10(4):283-90. doi: 10.1007/BF00365624.

DOI:10.1007/BF00365624
PMID:3916810
Abstract

Two cob- deletion mutants are characterized. One of them, M9410, is deleted for 911 bp of the noncoding sequences only which separate tRNAGlu and cob exon 1; it thus lacks most of the sequence encoding the 957 bp long cob leader (Bonitz et al. 1982) and some 20 bp 5' to it. The end points of this deletion coincide with 31 bp long direct repeats in wild type mtDNA. The other mutant, M9391, is deleted for all cob coding sequences and most of the cob leader sequence but it retains the 5' terminal 261 bp of this leader. Northern analysis revealed that M9410 totally lacks cob mRNA or pre-mRNA. The large deletion M9391 in contrast accumulates a 13S RNA which probably results from transcription through the junction, which ligates sequences of the cob leader to sequences of the cob-oli1 intergenic spacer.

摘要

对两个cob基因缺失突变体进行了表征。其中一个突变体M9410，仅缺失了位于tRNAGlu和cob外显子1之间的911 bp非编码序列；因此它缺少了编码957 bp长的cob前导序列的大部分序列（博尼茨等人，1982年）以及其5'端约20 bp的序列。该缺失的端点与野生型线粒体DNA中31 bp长的正向重复序列一致。另一个突变体M9391，缺失了所有cob编码序列和大部分cob前导序列，但保留了该前导序列5'端的261 bp。Northern分析表明，M9410完全缺乏cob mRNA或前体mRNA。相比之下，大缺失突变体M9391积累了一种13S RNA，这可能是由于转录通过了连接点，该连接点将cob前导序列与cob-oli1基因间隔区的序列连接起来。

相似文献

1
Deletions in the cob gene of yeast mtDNA and their phenotypic effect.酵母线粒体DNA中cob基因的缺失及其表型效应。
Curr Genet. 1985;10(4):283-90. doi: 10.1007/BF00365624.
2
The oli1 gene and flanking sequences in mitochondrial DNA of Saccharomyces cerevisiae: the complete nucleotide sequence of a 1.35 kilobase petite mitochondrial DNA genome covering the oli1 gene.酿酒酵母线粒体DNA中的oli1基因及其侧翼序列：一个覆盖oli1基因的1.35千碱基小线粒体DNA基因组的完整核苷酸序列。
Curr Genet. 1986;10(10):713-23. doi: 10.1007/BF00405093.
3
A GC cluster repeat is a hotspot for mit- macro-deletions in yeast mitochondrial DNA.GC 簇重复序列是酵母线粒体 DNA 中发生有丝分裂大缺失的热点区域。
Mol Gen Genet. 1991 Apr;226(1-2):233-40. doi: 10.1007/BF00273608.
4
Mitochondrial suppression of a yeast nuclear mutation which affects the translation of the mitochondrial apocytochrome b transcript.线粒体对酵母核突变的抑制作用，该核突变影响线粒体脱辅基细胞色素b转录本的翻译。
Curr Genet. 1985;9(8):641-8. doi: 10.1007/BF00449816.
5
Cytochrome b of cob revertants in yeast. 1. Isolation and characterization of revertants derived from cob exon mutants of Saccharomyces cerevisiae.酵母中cob回复突变体的细胞色素b。1. 酿酒酵母cob外显子突变体衍生回复突变体的分离与鉴定。
Mol Gen Genet. 1984;196(1):158-66. doi: 10.1007/BF00334109.
6
Deletion of the leader peptide of the mitochondrially encoded precursor of Saccharomyces cerevisiae cytochrome c oxidase subunit II.酿酒酵母细胞色素c氧化酶亚基II线粒体编码前体的前导肽缺失。
Genetics. 1997 Apr;145(4):903-10. doi: 10.1093/genetics/145.4.903.
7
Expression of the "split gene" cob in yeast mtDNA. Nuclear mutations specifically block the excision of different introns from its primary transcript.酵母线粒体DNA中“断裂基因” cob 的表达。核突变特异性地阻断了其初级转录本中不同内含子的切除。
J Biol Chem. 1983 Jul 10;258(13):7954-9.
8
In vivo analysis of sequences necessary for CBP1-dependent accumulation of cytochrome b transcripts in yeast mitochondria.酵母线粒体中细胞色素b转录本依赖CBP1积累所需序列的体内分析。
Mol Cell Biol. 1993 Jul;13(7):4203-13. doi: 10.1128/mcb.13.7.4203-4213.1993.
9
Two yeast nuclear genes, CBS1 and CBS2, are required for translation of mitochondrial transcripts bearing the 5'-untranslated COB leader.两个酵母核基因CBS1和CBS2是带有5'-非翻译COB前导序列的线粒体转录本翻译所必需的。
Curr Genet. 1986;11(1):41-5. doi: 10.1007/BF00389424.
10
Transmission of yeast mitochondrial loci to progeny is reduced when nearby intergenic regions containing ori sequences are deleted.当含有ori序列的邻近基因间区域被删除时，酵母线粒体基因座向后代的传递会减少。
Mol Gen Genet. 1988 Nov;214(3):425-32. doi: 10.1007/BF00330476.

引用本文的文献

1
In vivo analysis of sequences necessary for CBP1-dependent accumulation of cytochrome b transcripts in yeast mitochondria.酵母线粒体中细胞色素b转录本依赖CBP1积累所需序列的体内分析。
Mol Cell Biol. 1993 Jul;13(7):4203-13. doi: 10.1128/mcb.13.7.4203-4213.1993.
2
In vivo analysis of sequences required for translation of cytochrome b transcripts in yeast mitochondria.酵母线粒体中细胞色素b转录本翻译所需序列的体内分析。
Mol Cell Biol. 1995 Feb;15(2):780-9. doi: 10.1128/MCB.15.2.780.
3
Nuclearly-encoded CBP1 interacts with the 5' end of mitochondrial cytochrome b pre-mRNA.

本文引用的文献

1
Fine structure of OXI1, the mitochondrial gene coding for subunit II of yeast cytochrome c oxidase.酵母细胞色素 c 氧化酶亚基 II 编码基因 OXI1 的精细结构。
Curr Genet. 1979 Dec;1(1):75-83. doi: 10.1007/BF00413308.
2
The gene for the small ribosomal RNA on yeast mitochondrial DNA: Physical map, direction of transcription and absence of an intervening sequence.酵母线粒体 DNA 上小核糖体 RNA 基因：物理图谱、转录方向和无内含子。
Curr Genet. 1979 Dec;1(1):33-43. doi: 10.1007/BF00413305.
3
Physical map of the COB region in mitochondrial DNA of Saccharomyces cerevisiae.
细胞核编码的CBP1与线粒体细胞色素b前体mRNA的5'端相互作用。
Curr Genet. 1987;12(6):391-7. doi: 10.1007/BF00434815.
4
Site-specific AT-cluster insertions in the mitochondrial 15S rRNA gene of the yeast S. cerevisiae.酿酒酵母线粒体15S rRNA基因中的位点特异性AT簇插入
Nucleic Acids Res. 1988 Sep 12;16(17):8665-74. doi: 10.1093/nar/16.17.8665.
5
Putative target sites for mobile G + C rich clusters in yeast mitochondrial DNA: single elements and tandem arrays.酵母线粒体DNA中富含G + C的移动簇的假定靶位点：单个元件和串联阵列。
Mol Gen Genet. 1989 Aug;218(2):272-83. doi: 10.1007/BF00331278.
6
CBP1 function is required for stability of a hybrid cob-oli1 transcript in yeast mitochondria.酵母线粒体中杂种cob-oli1转录本的稳定性需要CBP1发挥功能。
Curr Genet. 1990 Dec;18(5):421-8. doi: 10.1007/BF00309911.
7
A GC cluster repeat is a hotspot for mit- macro-deletions in yeast mitochondrial DNA.GC 簇重复序列是酵母线粒体 DNA 中发生有丝分裂大缺失的热点区域。
Mol Gen Genet. 1991 Apr;226(1-2):233-40. doi: 10.1007/BF00273608.
酿酒酵母线粒体 DNA COB 区的物理图谱。
Curr Genet. 1981 Apr;3(1):65-71. doi: 10.1007/BF00419582.
4
Assembly of the mitochondrial membrane system. Processing of the apocytochrome b precursor RNAs in Saccharomyces cerevisiae D273-10B.线粒体膜系统的组装。酿酒酵母D273 - 10B中脱辅基细胞色素b前体RNA的加工。
J Biol Chem. 1982 Jun 10;257(11):6268-74.
5
Expression of the "split gene" COB in yeast mtDNA. Translation of intervening sequences in mutant strains.酵母线粒体DNA中“断裂基因”COB的表达。突变菌株中介入序列的翻译。
J Biol Chem. 1981 Apr 10;256(7):3525-31.
6
Assembly of the mitochondrial membrane system. CBP1, a yeast nuclear gene involved in 5' end processing of cytochrome b pre-mRNA.线粒体膜系统的组装。CBP1，一个参与细胞色素b前体mRNA 5'端加工的酵母核基因。
J Biol Chem. 1984 Apr 25;259(8):4722-31.
7
The mitochondrial genomes of spontaneous orir petite mutants of yeast have rearranged repeat units organized as inverted tandem dimers.酵母自发ori小菌落突变体的线粒体基因组具有重排的重复单元，这些重复单元组织成反向串联二聚体。
Gene. 1983 Sep;24(1):61-71. doi: 10.1016/0378-1119(83)90131-2.
8
Origins of transcripts of the yeast mitochondrial var 1 gene.酵母线粒体var 1基因转录本的起源
J Biol Chem. 1984 May 10;259(9):6019-27.
9
Identification of multiple transcriptional initiation sites on the yeast mitochondrial genome by in vitro capping with guanylyltransferase.通过用鸟苷酸转移酶进行体外加帽鉴定酵母线粒体基因组上的多个转录起始位点。
J Biol Chem. 1983 Nov 25;258(22):14025-33.
10
A nonanucleotide sequence involved in promotion of ribosomal RNA synthesis and RNA priming of DNA replication in yeast mitochondria.一种参与促进酵母线粒体核糖体RNA合成及DNA复制的RNA引发的九核苷酸序列。
Nucleic Acids Res. 1982 Dec 20;10(24):7993-8006. doi: 10.1093/nar/10.24.7993.