Weiller G F, Bruckner H, Kim S H, Pratje E, Schweyen R J
Institut für Genetik und Mikrobiologie, Universität München, FRG.
Mol Gen Genet. 1991 Apr;226(1-2):233-40. doi: 10.1007/BF00273608.
In a random collection of mit- mutations of the yeast strain 777-3A we find that deletions are exceptionally frequent in the OXI3 gene, a large mosaic gene coding for subunit I of cytochrome oxidase. About 10% of all oxi3-mutants carry the same macro-deletion, del-A, extending from the 5' non-translated leader of OXI3 to intron 5b of this gene. Determination of the respective wild-type sequences and of the del-A junction sequence revealed that the end-points of the deletion are in two GC clusters with 31 bp sequence identity which are located at a distance of 11.3 kb. We speculate that not only the sequence identity of the two GC clusters but also the palindromic structure of these putatively mobile elements of yeast mitochondrial DNA (mtDNA) plays a role in deletion formation.
在酵母菌株777 - 3A的随机线粒体（mit）突变集合中，我们发现，在OXI3基因（一个编码细胞色素氧化酶亚基I的大型镶嵌基因）中，缺失异常频繁。所有oxi3突变体中约10%携带相同的大缺失，即del - A，其范围从OXI3的5'非翻译前导区延伸至该基因的内含子5b。对相应野生型序列和del - A连接序列的测定表明，缺失的端点位于两个具有31 bp序列同一性的GC簇中，这两个GC簇相距11.3 kb。我们推测，不仅两个GC簇的序列同一性，而且酵母线粒体DNA（mtDNA）这些假定可移动元件的回文结构在缺失形成中都起作用。
