CINBIO, Universidade de Vigo, Departamento de Ingeniería Química, Campus Ourense, 32004 Ourense, Spain.
CITI-University of Vigo, Parque Tecnolóxico de Galicia, Rúa Galicia n° 2, 32900 Ourense, Spain.
Sci Total Environ. 2024 Nov 15;951:175654. doi: 10.1016/j.scitotenv.2024.175654. Epub 2024 Aug 20.
A simple cascade process based on the hydrothermal fractionation of Ulva spp. biomass was proposed. Considering the overall extraction yields (50 %), ulvan recovery (23 %), and ulvan composition, structural, mechanical and cytotoxic properties, the selected optimal final heating temperature was 160 °C. Ethanol precipitation provided the highest ulvan recovery yields but choline chloride precipitated ulvans showed stronger mechanical properties, G´ moduli 1.5·10 Pa and 3·10 Pa for ethanol and for choline chloride, respectively. Both products were safe on NCTC 929 mouse fibroblasts and after a cooling stage, formed films without requiring any additives. From the ulvan-free liquid fraction, one product with 43 % (wt, d.b.) phenolics and moderate antiradical properties and a byproduct containing nutrients and minerals were separated. The methane potential of the corresponding residual solids was influenced by the hydrothermal heating temperature and was doubled compared to than for the untreated seaweed biomass (60 mL/g VS). This scheme could be also applied to the wet algal biomass, in a chemical free alternative to provide ready to use ulvan biopolymers, bioactives, nutrients, salts and biogas, conforming a biorefinery approach.
提出了一种基于浒苔生物质水热分级的简单级联工艺。考虑到总提取率（50%）、岩藻聚糖回收率（23%）以及岩藻聚糖的组成、结构、力学和细胞毒性特性，选择的最佳最终加热温度为 160°C。乙醇沉淀提供了最高的岩藻聚糖回收率，但氯化胆碱沉淀的岩藻聚糖表现出更强的力学性能，G´模量分别为 1.5·10 Pa 和 3·10 Pa。这两种产品对 NCTC 929 小鼠成纤维细胞均安全，冷却后无需添加任何添加剂即可形成薄膜。从无岩藻聚糖的液体部分中，分离出一种具有 43%（wt，干重）酚类物质和适度自由基清除能力的产品，以及一种含有营养物质和矿物质的副产物。相应的残余固体的甲烷潜力受到水热加热温度的影响，与未经处理的海藻生物质相比增加了一倍（60 mL/g VS）。该方案也可应用于湿海藻生物质，为提供即用型岩藻聚糖生物聚合物、生物活性物质、营养物质、盐和沼气提供了一种无化学物质的替代方法，符合生物炼制方法。
