Saponenko Vadim, Nazhmetdinov Azamat
Test Laboratory, LLP "TAU Scientific and Production Association", Almaty, Republic of Kazakhstan.
Scientific Department, LLP "TAU Scientific and Production Association", Almaty, Republic of Kazakhstan.
J Pharm Bioallied Sci. 2024 Apr-Jun;16(2):60-65. doi: 10.4103/jpbs.jpbs_85_24. Epub 2024 Jul 15.
Pesticides are actively used in agriculture to protect crops from pests, and accurate determination of the concentration of their active ingredient is critical to ensuring their efficacy and safety. The research aims to develop a methodology that will enable the accurate determination of the mass concentration of diflubenzuron, a widely used active ingredient in pesticide formulations. This is achieved through the use of high-performance liquid chromatography (HPLC).
The proposed method for determining the mass concentration of diflubenzuron using HPLC was developed through a series of experiments and optimisation steps. The sample preparation procedure was optimised to extract the maximum amount of diflubenzuron from the pesticide formulation.
The chromatography conditions were optimised to separate diflubenzuron from other components in the sample matrix. A calibration approach was established for accurate quantification of diflubenzuron in the sample based on peak area measurements. The developed method can be widely used in agriculture, where accurate determination of diflubenzuron concentrations in pesticide formulations is critical for proper application and regulatory compliance. Both regulatory organizations and pesticide manufacturers can use it for batch-release testing and quality control to evaluate the efficacy and safety of pesticide products. In addition, the method can serve as a valuable tool for researchers and analysts working with pesticide residues in crops and environmental monitoring studies.
The optimised sample preparation, chromatography conditions, and calibration approach provide reliable and reproducible results.
农药在农业中被广泛用于保护作物免受害虫侵害，准确测定其活性成分的浓度对于确保其有效性和安全性至关重要。本研究旨在开发一种方法，能够准确测定农药制剂中广泛使用的活性成分敌草隆的质量浓度。这是通过使用高效液相色谱法（HPLC）实现的。
通过一系列实验和优化步骤，开发了使用HPLC测定敌草隆质量浓度的方法。优化了样品制备程序，以从农药制剂中提取最大量的敌草隆。
优化了色谱条件，以将敌草隆与样品基质中的其他成分分离。建立了一种校准方法，基于峰面积测量对样品中的敌草隆进行准确定量。所开发的方法可广泛应用于农业领域，在该领域中，准确测定农药制剂中敌草隆的浓度对于正确施用和符合监管要求至关重要。监管机构和农药制造商均可将其用于批放行测试和质量控制，以评估农药产品的有效性和安全性。此外，该方法可为从事作物中农药残留研究和环境监测研究的研究人员和分析人员提供有价值的工具。
优化后的样品制备、色谱条件和校准方法提供了可靠且可重复的结果。
