Bioengineering Graduate Program, University of Kansas, Lawrence, KS, United States.
Institute for Bioengineering Research, University of Kansas, Lawrence, KS, United States.
Exp Biol Med (Maywood). 2024 Aug 7;249:10096. doi: 10.3389/ebm.2024.10096. eCollection 2024.
The current study explores the potential of ultrasound-assisted laser therapy (USaLT) to selectively destroy melanoma cells. The technology was tested on an melanoma model, which was established by growing melanoma cells in chicken breast tissue. Ultrasound-only and laser-only treatments were used as control groups. USaLT was able to effectively destroy melanoma cells and selectively remove 66.41% of melanoma cells in the tumor model when an ultrasound peak negative pressure of 2 MPa was concurrently applied with a laser fluence of 28 mJ/cm at 532 nm optical wavelength for 5 min. The therapeutic efficiency was further improved with the use of a higher laser fluence, and the treatment depth was improved to 3.5 mm with the use of 1,064 nm laser light at a fluence of 150 mJ/cm. None of the laser-only and ultrasound-only treatments were able to remove any melanoma cells. The treatment outcome was validated with histological analyses and photoacoustic imaging. This study opens the possibility of USaLT for melanoma that is currently treated by laser therapy, but at a much lower laser fluence level, hence improving the safety potential of laser therapy.
本研究探索了超声辅助激光治疗（USaLT）选择性破坏黑色素瘤细胞的潜力。该技术在鸡胸脯组织中生长黑色素瘤细胞建立的黑色素瘤模型上进行了测试。超声治疗和激光治疗分别作为对照组。当在 532nm 光波长下应用 2MPa 的超声峰负压并同时应用 28mJ/cm 的激光能量持续 5 分钟时，USaLT 能够有效破坏黑色素瘤细胞，并选择性去除肿瘤模型中 66.41%的黑色素瘤细胞。使用更高的激光能量可以进一步提高治疗效率，并且使用 1064nm 激光在 150mJ/cm 的能量下可以将治疗深度提高到 3.5mm。激光治疗和超声治疗均无法去除任何黑色素瘤细胞。通过组织学分析和光声成像验证了治疗效果。本研究为目前采用激光治疗的黑色素瘤开辟了 USaLT 的可能性，但激光能量水平要低得多，从而提高了激光治疗的安全性。
