单细胞测序分析显示，WDR72是胃癌中一个新的与癌症干细胞相关的基因。
Single-cell sequencing analysis revealed that WDR72 was a novel cancer stem cells related gene in gastric cancer.
作者信息
Zheng Lei, Lu Jia, Kong Dalu, Zhan Yang
机构信息
Department of Colorectal Oncology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Key Laboratory of Digestive Cancer, Tianjin, 300060, China.
Department of Infection Management, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Key Laboratory of Digestive Cancer, Tianjin, 300060, China.
出版信息
Heliyon. 2024 Jul 31;10(15):e35549. doi: 10.1016/j.heliyon.2024.e35549. eCollection 2024 Aug 15.
BACKGROUND
Cancer stem cells (CSCs) are pivotal in tumor resistance to chemotherapy and gastric cancer's rapid proliferation and metastasis. We aimed to explore the CSCs-related genes in gastric cancer epithelial cells.
METHODS
The mRNA expression profile and single-cell sequencing data of gastric cancer were downloaded from the public database.
RESULTS
We identified WDR72 as a CSCs-related gene in gastric cancer epithelial cells. WDR72 was highly expressed in gastric cancer tissues, and high expression of WDR72 was associated with inferior prognosis of patients. WDR72 expression had a significant negative correlation with the infiltration of CD8 + T cells and activated memory CD4 + T cells. PD-L1 expression was significantly reduced in gastric cancer patients with high WDR72 expression. WDR72 was correlated with IC50 of multiple small-molecule drugs.
CONCLUSION
We identified a novel CSCs-related gene in gastric cancer epithelial cells, WDR72, which was highly expressed in patients with high stemness scores.
背景
癌症干细胞（CSCs）在肿瘤对化疗的抗性以及胃癌的快速增殖和转移中起关键作用。我们旨在探索胃癌上皮细胞中与CSCs相关的基因。
方法
从公共数据库下载胃癌的mRNA表达谱和单细胞测序数据。
结果
我们鉴定出WDR72是胃癌上皮细胞中与CSCs相关的基因。WDR72在胃癌组织中高表达，且WDR72的高表达与患者预后较差相关。WDR72表达与CD8 + T细胞和活化记忆CD4 + T细胞的浸润呈显著负相关。WDR72高表达的胃癌患者中PD-L1表达显著降低。WDR72与多种小分子药物的IC50相关。
结论
我们在胃癌上皮细胞中鉴定出一个新的与CSCs相关的基因WDR72，其在干性评分高的患者中高表达。
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