Elhasid Ronit, Baron Szilvia, Fidel Victoria, Kaganov Kira, Shukrun Rachel
Pediatric Hemato-Oncology Research Laboratory, Tel Aviv Medical Center, Tel Aviv, Israel.
Department of Pediatric Hemato-Oncology, Tel Aviv Medical Center, Tel Aviv, Israel.
Heliyon. 2024 Jul 30;10(15):e35470. doi: 10.1016/j.heliyon.2024.e35470. eCollection 2024 Aug 15.
Resident physicians on long-term night shifts often face sleep deprivation, affecting the immune response, notably neutrophils, vital to innate defense mechanisms. Sleep-deprived residents exhibit altered neutrophil counts and reduced phagocytosis and NADPH oxidase activity, critical to combating infections. Our study focused on neutrophil extracellular traps (NETs), a defense process against pathogens not previously linked to sleep loss. Results revealed that sleep-deprived residents exhibited a 19.8 % reduction in NET formation compared to hospital workers with regular sleep patterns (P < 0.01). Additionally, key NETs proteins, Neutrophil Elastase and Myeloperoxidase, were less active in sleep-deprived individuals (1.53mU; P < 0.01 and 0.95U; P < 0.001 decrease, accordingly). Interestingly, the ability to form NETs resumed to normal levels three months post-residency among pediatric residents. The causal relationship between reduced NETs due to sleep deprivation and the increased susceptibility to infections, as well as its implications for infection severity, is a critical area for further investigation.
长期值夜班的住院医师经常面临睡眠不足的问题，这会影响免疫反应，尤其是对先天防御机制至关重要的中性粒细胞。睡眠不足的住院医师中性粒细胞计数改变，吞噬作用和烟酰胺腺嘌呤二核苷酸磷酸氧化酶活性降低，而这些对于抵抗感染至关重要。我们的研究聚焦于中性粒细胞胞外诱捕网（NETs），这是一种此前未与睡眠不足相关联的抵御病原体的过程。结果显示，与睡眠模式正常的医院工作人员相比，睡眠不足的住院医师NETs形成减少了19.8%（P < 0.01）。此外，关键的NETs蛋白，中性粒细胞弹性蛋白酶和髓过氧化物酶，在睡眠不足的个体中活性较低（分别降低了1.53mU；P < 0.01和0.95U；P < 0.001）。有趣的是，儿科住院医师在住院实习结束三个月后，形成NETs的能力恢复到了正常水平。睡眠不足导致NETs减少与感染易感性增加之间的因果关系，以及其对感染严重程度的影响，是需要进一步研究的关键领域。
