Ahmed M Yahya, Cakir Muharrem Okan, Salman Nadia Aziz, Sandhu Sarbjinder, Ashrafi G Hossein
School of Life Science, Pharmacy and Chemistry, Kingston University London, London, KT1 2EE, UK.
Department of Urology and Surgery, Kingston Hospital, Kingston Upon Thames, London, KT2 7QB, UK.
Heliyon. 2024 Jul 24;10(15):e35074. doi: 10.1016/j.heliyon.2024.e35074. eCollection 2024 Aug 15.
Human papillomavirus (HPV) infections, primarily transmitted through sexual contact, have been linked to various cancers, including cervical, penile, anal, oropharynx, breast, and prostate cancers. This study presents a unique case of concurrent high-risk HPV35, HPV45, and HPV59 infections in both prostate and bladder cancer tissues from a single patient, representing the first documented instance worldwide with identical HPV types detected in two adjacent organs of the same individual. Employing a multiplex-PCR approach, gel electrophoresis, and Sanger sequencing, we confirmed the presence of these high-risk HPV types. Additionally, Western blot analysis using an HPV E7 antibody demonstrated the active expression of HPV oncoproteins in both cancer types. This discovery underscores the potential for HPV intra-organ transmission and necessitates further exploration of alternative transmission routes. The implications of our results offer new insights into the complex dynamics of HPV transmission in cancer pathogenesis. In conclusion our study reveals concurrent HPV infections in both prostate and bladder cancers within a single patient and highlights the potential intra-organ spread of HPV and the need for further investigation of alternative transmission routes.
人乳头瘤病毒（HPV）感染主要通过性接触传播，与多种癌症有关，包括宫颈癌、阴茎癌、肛门癌、口咽癌、乳腺癌和前列腺癌。本研究报告了一例独特病例，一名患者的前列腺癌和膀胱癌组织中同时存在高危型HPV35、HPV45和HPV59感染，这是全球首例在同一个体的两个相邻器官中检测到相同HPV类型的记录实例。我们采用多重聚合酶链反应（multiplex-PCR）方法、凝胶电泳和桑格测序，证实了这些高危型HPV的存在。此外，使用HPV E7抗体的蛋白质免疫印迹分析表明，这两种癌症类型中均有HPV癌蛋白的活性表达。这一发现强调了HPV在器官内传播的可能性，有必要进一步探索其他传播途径。我们的研究结果为HPV在癌症发病机制中的复杂传播动态提供了新的见解。总之，我们的研究揭示了一名患者的前列腺癌和膀胱癌中同时存在HPV感染，并强调了HPV在器官内传播的可能性以及进一步研究其他传播途径的必要性。
