Rios-Ruiz Winston Franz, Tuanama-Reátegui Ciceron, Huamán-Córdova Gamaniel, Valdez-Nuñez Renzo Alfredo
Laboratorio de Microbiología Agrícola, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, Perú.
Departamento Académico de Ciencias Básicas, Facultad de Ingeniería, Universidad Nacional de Barranca, Barranca 15169, Perú.
Plants (Basel). 2023 Jan 23;12(3):524. doi: 10.3390/plants12030524.
Multiple biotic and abiotic factors influence rice cultivation. These factors limit productivity and yield, as well as an irrational use of agrochemicals in rice cultivation. A sustainable alternative is using selected growth-promoting microorganisms to increase nutritional efficiency. In the present study, the direct mechanisms of growth promotion in two strains of , three strains of and two strains of endophytes of rice were characterized. TUR07-02b and SMBH14-02 were selected to promote Oryza sativa var's growth. "Bellavista" was used at different doses (50, 75, and 100%) of mineral nitrogen (N) using a randomized block design by quintuplicate. Both strains, SMBH14-02 and TUR07-02b, presented outstanding promoter characteristics, including auxin production (123.17 and 335.65 μg mL, respectively) and biological nitrogen fixation capacity. Similarly, TUR07-02b could solubilize phosphate-Ca (20.94 μg mL), cellulases, and pectinases. Under greenhouse conditions, co-inoculated plants receiving 75% of the total dose of mineral nitrogen showed increased agronomic parameters in relation to panicle length, grains per panicle, grain yield, and harvest index by 25.0, 30.7, 39.5, and 12.5%, respectively, compared to the 75% fertilized treatment without inoculation. The strains of TUR07-02b and SMBH14-02 are potential microbial resources in the formulation of new inoculants to reduce the use of nitrogenous fertilizers. Thus, agronomic validation of the inoculant consortium at the field level will be an essential step in providing an alternative for the sustainable management of rice cultivation and increased productivity of rice farmers in the San Martín region.
多种生物和非生物因素影响水稻种植。这些因素限制了生产力和产量，以及水稻种植中农用化学品的不合理使用。一种可持续的替代方法是使用选定的促生长微生物来提高营养效率。在本研究中，对两株水稻内生菌、三株水稻内生菌和两株水稻内生菌的促生长直接机制进行了表征。选择TUR07-02b和SMBH14-02来促进水稻品种的生长。采用随机区组设计，重复五次，以不同剂量（50%、75%和100%）的矿质氮（N）处理“贝拉维斯塔”。SMBH14-02和TUR07-02b这两个菌株都表现出优异的促生长特性，包括生长素产生（分别为123.17和335.65μg/mL）和生物固氮能力。同样，TUR07-02b可以溶解磷酸钙（20.94μg/mL）、纤维素酶和果胶酶。在温室条件下，与未接种的75%施肥处理相比，接种了75%总剂量矿质氮的共接种植物在穗长、每穗粒数、籽粒产量和收获指数方面的农艺参数分别提高了25.0%、30.7%、39.5%和12.5%。TUR07-02b和SMBH14-02菌株是新型接种剂配方中潜在的微生物资源，可减少氮肥的使用。因此，在田间水平对接种剂组合进行农艺验证将是为圣马丁地区水稻种植的可持续管理和提高稻农生产力提供替代方案的关键一步。
