Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Vet Res Commun. 2024 Dec;48(6):3593-3603. doi: 10.1007/s11259-024-10501-1. Epub 2024 Aug 22.
Porcine circovirus type 3 (PCV3) infection is clinically related to various diseases, including porcine dermatitis and nephrotic syndrome (PDNS)-like disease, respiratory disease, reproductive disorders, and gastrointestinal and neurological diseases. Since PCV3 infection was discovered in 2016, it has developed rapidly and has attracted much attention worldwide. However, specific preventive and therapeutic interventions are currently lacking. In this study, four-dimensional (4D) data-independent acquisition (DIA)-based quantitative proteomics detection combined with bioinformatics analysis were employed to quantitatively identify the differentially expressed proteins in PK-15 cells from the PCV3-infected group compared with those from the uninfected control group. A total of 194 cellular proteins were significantly altered in response to PCV3 infection, including 58 upregulated proteins and 136 downregulated proteins. In our Gene Ontology (GO) enrichment analysis, these differentially expressed proteins were mostly associated with cellular anatomical entities, binding, cellular processes, biological regulation, catalytic activity, metabolic processes, developmental processes, protein-containing complexes and responses to stimuli. Our Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEPs were predominantly involved in metabolic pathways, the cAMP signaling pathway, protein processing in the endoplasmic reticulum, the PI3K-Akt signaling pathway, and the calcium signaling pathway. For the experiments, Western blotting (WB) was used to confirm the changes in important molecules. The differentially expressed proteins identified should contribute to a greater understanding of the mechanism of PCV3 replication and pathogenesis, as well as the host response.
猪圆环病毒 3 型（PCV3）感染与多种疾病有关，包括猪皮炎肾病综合征（PDNS）样疾病、呼吸道疾病、生殖障碍以及胃肠道和神经系统疾病。自 2016 年发现 PCV3 感染以来，它在全球范围内迅速发展并引起了广泛关注。然而，目前尚无特定的预防和治疗干预措施。在本研究中，采用基于四维（4D）无依赖数据采集（DIA）的定量蛋白质组学检测结合生物信息学分析，定量鉴定了 PCV3 感染的 PK-15 细胞与未感染对照细胞之间的差异表达蛋白。共鉴定到 194 种细胞蛋白对 PCV3 感染有显著变化，其中 58 种蛋白上调，136 种蛋白下调。在我们的基因本体论（GO）富集分析中，这些差异表达蛋白主要与细胞解剖实体、结合、细胞过程、生物调节、催化活性、代谢过程、发育过程、含蛋白复合物和对刺激的反应有关。我们的京都基因与基因组百科全书（KEGG）分析显示，差异表达蛋白主要参与代谢途径、cAMP 信号通路、内质网中的蛋白加工、PI3K-Akt 信号通路和钙信号通路。对于实验，我们使用 Western blot（WB）来验证重要分子的变化。鉴定出的差异表达蛋白应该有助于更好地理解 PCV3 复制和发病机制以及宿主反应的机制。
