Wang Cheng, Zhang Jing, Lv Jian, Li Jing, Gao Yanqiang, Patience Bakpa Emily, Niu Tianhang, Yu Jihua, Xie Jianming
College of Horticulture, Gansu Agricultural University, Lanzhou, China.
Front Nutr. 2022 Apr 5;9:859035. doi: 10.3389/fnut.2022.859035. eCollection 2022.
Hydroponic culture has become a commercial planting model for leafy vegetables, herbs, and other plants with medicinal value. Methyl jasmonate (MeJA) is involved in primary and secondary plant metabolism; moreover, it regulates plant bioactive compounds and enhances the nutritional and medicinal value of plants. We performed targeted metabolomic analysis of the primary and secondary metabolites in substrate-grown and hydroponic Chinese chive leaves sprayed with MeJA (0, 300, 500, and 800 μM). Using ultra-performance liquid chromatography (UPLC), UPLC tandem mass spectrometry, and chemometric tools, and analyzed the antioxidant activity of these plants. We identified the biomarkers of amino acids (serine, proline, lysine, and arginine) and phenolic compounds (4-coumaric acid and protocatechuic acid) using chemometric tools to distinguish between substrate-grown and hydroponic Chinese chives treated with MeJA. MeJA (500 μM) treatment significantly increased the total sugar and amino acid (essential and non-essential amino acids and sulfur-containing amino acids) contents of hydroponically grown Chinese chives. However, the changes in total sugar and amino acid contents in Chinese chive grown in substrates showed the opposite trend. The organic acid content of hydroponically grown Chinese chives treated with MeJA decreased significantly, whereas that of substrate-grown plants treated with 300 μM MeJA increased significantly. Further, MeJA treatment significantly increased the phenolic content of substrate-grown Chinese chives. Treatment with 800 μM MeJA significantly increased the carotenoid content of substrate-grown Chinese chives and the phenolic content of hydroponic Chinese chives. In addition, the 500 μM MeJA treatment significantly increased the antioxidant activity of Chinese chives in both substrate-grown and hydroponic cultures, and promoted the accumulation of nutrients and bioactive substances. This treatment also improved the flavor quality of these plants and their nutritional and medicinal value. Thus, the results suggested that MeJA-treated plants could be used as value-added horticultural products.
水培已成为叶菜类蔬菜、香草及其他具有药用价值植物的一种商业种植模式。茉莉酸甲酯（MeJA）参与植物的初级和次级代谢；此外，它还能调节植物生物活性化合物，并提高植物的营养和药用价值。我们对喷施了MeJA（0、300、500和800 μM）的基质栽培和水培韭菜叶片中的初级和次级代谢产物进行了靶向代谢组学分析。使用超高效液相色谱（UPLC）、UPLC串联质谱和化学计量工具，并分析了这些植物的抗氧化活性。我们使用化学计量工具鉴定了氨基酸（丝氨酸、脯氨酸、赖氨酸和精氨酸）和酚类化合物（对香豆酸和原儿茶酸）的生物标志物，以区分喷施MeJA的基质栽培和水培韭菜。500 μM MeJA处理显著增加了水培韭菜的总糖和氨基酸（必需氨基酸、非必需氨基酸和含硫氨基酸）含量。然而，基质栽培韭菜中总糖和氨基酸含量的变化趋势相反。喷施MeJA的水培韭菜的有机酸含量显著降低，而喷施300 μM MeJA的基质栽培植物的有机酸含量显著增加。此外，MeJA处理显著增加了基质栽培韭菜的酚类含量。800 μM MeJA处理显著增加了基质栽培韭菜的类胡萝卜素含量和水培韭菜的酚类含量。此外，500 μM MeJA处理显著增加了基质栽培和水培韭菜的抗氧化活性，并促进了营养物质和生物活性物质的积累。这种处理还改善了这些植物的风味品质及其营养和药用价值。因此，结果表明，经MeJA处理的植物可作为增值园艺产品。
