大鼠脑中肽组氨酸异亮氨酸酰胺-27样肽的亚细胞分布及其在体外从大鼠大脑皮质切片中的释放。

The subcellular distribution of peptide histidine isoleucine amide-27-like peptides in rat brain and their release from rat cerebral cortical slices in vitro.

作者信息

Korchak D M, Gysling K, Beinfeld M C

出版信息

J Neurochem. 1985 Jan;44(1):255-9. doi: 10.1111/j.1471-4159.1985.tb07138.x.

DOI:10.1111/j.1471-4159.1985.tb07138.x

Abstract

The subcellular distribution of peptide histidine isoleucine amide (PHI)-27-like peptides (PLP) was investigated in rat cerebral cortex and whole rat brain in comparison with the distribution of vasoactive intestinal peptide (VIP). The highest content of PLP was found in the crude mitochondrial fraction (P2) and was also detected in the microsomal pellet. PLP was recovered in synaptosomes when further fractionation of P2 was performed. This distribution of PLP closely follows that of VIP and is suggestive of possible storage in vesicles at the nerve terminal. Basal release of PLP from rat cerebral cortical slices was below the detection limit of the PHI radioimmunoassay. However, depolarization by 55 mM potassium induced measurable PLP release. This release was calcium-dependent. These findings support the hypothesis that PLP could play a role in neurotransmission.

摘要

与血管活性肠肽（VIP）的分布相比，研究了大鼠大脑皮层和全脑中肽组氨酸异亮氨酸酰胺（PHI）-27样肽（PLP）的亚细胞分布。在粗线粒体部分（P2）中发现PLP含量最高，在微粒体沉淀中也检测到了PLP。当对P2进行进一步分级分离时，在突触小体中回收了PLP。PLP的这种分布与VIP的分布密切相关，提示可能储存在神经末梢的囊泡中。大鼠大脑皮层切片中PLP的基础释放低于PHI放射免疫测定的检测限。然而，55 mM钾诱导的去极化导致可测量的PLP释放。这种释放是钙依赖性的。这些发现支持了PLP可能在神经传递中发挥作用的假说。