State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Guizhou Panzheng Agriculture Ltd., PR China; National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, PR China; The Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, PR China.
Int J Biol Macromol. 2024 Jun;270(Pt 2):132222. doi: 10.1016/j.ijbiomac.2024.132222. Epub 2024 May 8.
Fungal polysaccharides have been explored by many for both structural studies and biological activities, but few studies have been done on the extracellular polysaccharides of Dictyophora rubrovalvata, so a new exopolysaccharide was isolated from Dictyophora rubrovalvata and its structure and its immunological activity were investigated. The crude exopolysaccharide (EPS) was purified by DEAE52 cellulose and Sephadex G-200 to obtain a new acidic polysaccharide (DR-EPS). DR-EPS (2.66 × 10 kDa) was consisted mainly of mannose, glucose, galactose and glucuronic acid with a molar ratio of 1: 0.86: 0.20: 0.01. In addition, DR-EPS increased the phagocytic activity of RAW264.7 cells up to 2.67 times of the blank control group. DR-EPS improved intracellular nucleic acid and glycogen metabolism as observed by AO and PAS staining. DR-EPS(40 μg/mL) promoted NO production up to 30.66 μmol, enhanced acid phosphatase (ACP) and superoxide dismutase (SOD) activities, with activity maxima of 660 U/gprot and 96.27 U/mgprot, respectively, and DR-EPS (160 μg / mL) significantly increased the lysozyme content as 2.73 times of the control group. The good immunological activity of extracellular polysaccharides of Dictyophora rubrovalvata provides directions for the use of fermentation broths.
真菌多糖已被许多人用于结构研究和生物活性研究，但对红缘拟层孔菌的胞外多糖研究甚少，因此，从红缘拟层孔菌中分离出一种新的胞外多糖，并对其结构和免疫活性进行了研究。粗胞外多糖（EPS）通过 DEAE52 纤维素和 Sephadex G-200 纯化，得到一种新的酸性多糖（DR-EPS）。DR-EPS（2.66×10 kDa）主要由甘露糖、葡萄糖、半乳糖和葡萄糖醛酸组成，摩尔比为 1：0.86：0.20：0.01。此外，DR-EPS 使 RAW264.7 细胞的吞噬活性提高至空白对照组的 2.67 倍。通过 AO 和 PAS 染色观察到，DR-EPS 改善了细胞内核酸和糖原代谢。DR-EPS（40μg/mL）可使 NO 生成量增加至 30.66μmol，增强酸性磷酸酶（ACP）和超氧化物歧化酶（SOD）活性，最大活性分别为 660 U/gprot 和 96.27 U/mgprot，DR-EPS（160μg/mL）可使溶菌酶含量显著增加至对照组的 2.73 倍。红缘拟层孔菌胞外多糖具有良好的免疫活性，为发酵液的利用提供了方向。
