Khasapane Ntelekwane George, Nkhebenyane Jane, Mnisi Zamantungwa, Kwenda Stanford, Thekisoe Oriel
Department of Life Sciences, Centre for Applied Food Safety and Biotechnology, Central University of Technology, Bloemfontein, South Africa.
Clinvet International, Study Operations, Bloemfontein, South Africa.
Front Microbiol. 2024 Apr 8;15:1376620. doi: 10.3389/fmicb.2024.1376620. eCollection 2024.
species are the primary cause of mastitis in dairy cows across the world. has recently become a pathogen that is zoonotic and multidrug resistant. This study aimed to sequence whole genomes of 38 isolates from 55 subclinical mastitis dairy cows of 7 small-scale farmers in the Free State Province, South Africa and document and their antimicrobial and virulence genes. The 38 isolates were grouped by the multi-locus sequencing types (MLST) into seven sequence types (STs), that is (ST 97, 352, 152, 243) and three new STs (ST8495, ST8500, and ST8501). Thirty-three isolates were divided into 7 core single-nucleotide polymorphism (SNP) clusters. Among the 9 distinct that were detected, t2883 accounted for the majority of isolates at 12 (31.57%), followed by t416 with 11 (28.94%) and t2844 with 5 (13.15%). The data also revealed the identification of four (4) plasmids, with Rep_N (rep20) accounting for the majority of isolates with 17 (44.73%), followed by Inc18 (repUS5) with 2 (5.26%). These isolates included 11 distinct antimicrobial resistance genes and 23 genes linked to bacterial virulence. Surprisingly, no methicillin resistance associated genes were detected in these isolates. Genome data of the current study will contribute to understanding epidemiology genotypes and ultimately aid in developing treatment and control plans to stop the spread of mastitis in the Free State province and South Africa as a whole.
该物种是全球奶牛乳腺炎的主要病因。最近它已成为一种具有人畜共患病性和多重耐药性的病原体。本研究旨在对来自南非自由邦省7个小规模养殖户的55头亚临床乳腺炎奶牛的38株分离株进行全基因组测序，并记录其抗菌基因和毒力基因。这38株分离株通过多位点测序类型（MLST）被分为7种序列类型（STs），即（ST 97、352、152、243）和3种新的STs（ST8495、ST8500和ST8501）。33株分离株被分为7个核心单核苷酸多态性（SNP）簇。在检测到的9种不同类型中，t2883占分离株的大多数，为12株（31.57%），其次是t416，有11株（28.94%），t2844有5株（13.15%）。数据还显示鉴定出了4种质粒，其中Rep_N（rep20）占分离株的大多数，为17株（44.73%），其次是Inc18（repUS5），有2株（5.26%）。这些分离株包括11种不同的抗菌抗性基因和23种与细菌毒力相关的基因。令人惊讶的是，在这些分离株中未检测到与耐甲氧西林相关的基因。本研究的基因组数据将有助于了解流行病学基因型，并最终有助于制定治疗和控制计划，以阻止乳腺炎在自由邦省乃至整个南非的传播。
