• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于毛细管电泳与质谱检测联用的小型化纳米电喷雾接口。

Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection.

作者信息

Václavek Tomáš, Vereshchagina Elizaveta, Nazareno Leny, Summanwar Anand, Foret František, Řemínek Roman

机构信息

Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czechia.

Department of Smart Sensors and Microsystems, SINTEF Digital, Oslo, Norway.

出版信息

Electrophoresis. 2024 Nov;45(21-22):1988-1994. doi: 10.1002/elps.202400090. Epub 2024 Aug 23.

DOI:10.1002/elps.202400090
PMID:39177276
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11662199/
Abstract

A miniaturized electrospray interface consisting of a microfluidic nanosprayer and nanospray module is reported in the presented short communication. The nanosprayer was fabricated using silicon (Si) technology suitable for cost-efficient high-volume mass production. The nanospray module enabled the positioning of the nanosprayer in front of a mass spectrometry entrance and its coupling with capillary electrophoresis based on the liquid junction principle. A case study of top-down and bottom-up proteomic analyses of intact cytochrome c and its tryptic digest demonstrates the practical applicability of the developed interface.

摘要

在本简短通讯中报道了一种由微流控纳米喷雾器和纳米喷雾模块组成的小型化电喷雾接口。该纳米喷雾器采用适用于具有成本效益的大批量生产的硅（Si）技术制造。纳米喷雾模块能够将纳米喷雾器定位在质谱入口前，并基于液接原理将其与毛细管电泳耦合。对完整细胞色素c及其胰蛋白酶消化产物进行自上而下和自下而上蛋白质组分析的案例研究证明了所开发接口的实际适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/2a2098bdc745/ELPS-45--g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/6de6b3e2def1/ELPS-45--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/6367de4d9f7b/ELPS-45--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/9b8315273a09/ELPS-45--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/2a2098bdc745/ELPS-45--g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/6de6b3e2def1/ELPS-45--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/6367de4d9f7b/ELPS-45--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/9b8315273a09/ELPS-45--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68b6/11662199/2a2098bdc745/ELPS-45--g004.jpg

相似文献

1
Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection.用于毛细管电泳与质谱检测联用的小型化纳米电喷雾接口。
Electrophoresis. 2024 Nov;45(21-22):1988-1994. doi: 10.1002/elps.202400090. Epub 2024 Aug 23.
2
Design and performance of a sheathless capillary electrophoresis/mass spectrometry interface by combining fused-silica capillaries with gold-coated nanoelectrospray tips.通过将熔融石英毛细管与金涂层纳米电喷雾尖端相结合设计并实现无鞘毛细管电泳/质谱联用接口的性能
Rapid Commun Mass Spectrom. 2005;19(7):881-5. doi: 10.1002/rcm.1866.
3
A disposable poly(methylmethacrylate)-based microfluidic module for protein identification by nanoelectrospray ionization-tandem mass spectrometry.一种基于聚甲基丙烯酸甲酯的一次性微流控模块，用于通过纳米电喷雾电离串联质谱法进行蛋白质鉴定。
Electrophoresis. 2001 Oct;22(18):3972-7. doi: 10.1002/1522-2683(200110)22:18<3972::AID-ELPS3972>3.0.CO;2-6.
4
Modified liquid junction interface for nonaqueous capillary electrophoresis-mass spectrometry.用于非水毛细管电泳-质谱联用的改进型液接界面
Electrophoresis. 2000 Sep;21(15):3311-7. doi: 10.1002/1522-2683(20000901)21:15<3311::AID-ELPS3311>3.0.CO;2-N.
5
Capillary electrophoresis in an extended nanospray tip-electrospray as an electrophoretic column.在扩展的纳米喷雾尖端电喷雾中作为电泳柱的毛细管电泳。
J Chromatogr A. 2015 Apr 3;1388:274-9. doi: 10.1016/j.chroma.2015.02.042. Epub 2015 Feb 21.
6
Tapered-Tip Capillary Electrophoresis Nano-Electrospray Ionization Mass Spectrometry for Ultrasensitive Proteomics: the Mouse Cortex.锥形尖端毛细管电泳-纳喷雾电喷雾电离质谱法用于超灵敏蛋白质组学研究：以小鼠大脑皮层为例。
J Am Soc Mass Spectrom. 2017 Apr;28(4):597-607. doi: 10.1007/s13361-016-1532-8. Epub 2016 Nov 16.
7
Two capillary approach for a multifunctional nanoflow sheath liquid interface for capillary electrophoresis-mass spectrometry.两种用于毛细管电泳-质谱的多功能纳米流鞘液界面的毛细管口进样方法。
Electrophoresis. 2021 Feb;42(4):369-373. doi: 10.1002/elps.202000169. Epub 2020 Aug 19.
8
Bottom-up proteomics of Escherichia coli using dynamic pH junction preconcentration and capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry.使用动态pH连接预浓缩和毛细管区带电泳-电喷雾电离-串联质谱法对大肠杆菌进行自下而上的蛋白质组学研究。
Anal Chem. 2014 Jul 1;86(13):6331-6. doi: 10.1021/ac5004486. Epub 2014 Jun 9.
9
[Analysis of recombinant erythropoietin and its tryptic digest by capillary electrophoresis and capillary electrophoresis-electrospray ionization-mass spectrometry using capillary coated with 6,6-ionene].[使用涂覆有6,6-紫罗烯的毛细管通过毛细管电泳和毛细管电泳-电喷雾电离-质谱法分析重组促红细胞生成素及其胰蛋白酶消化产物]
Se Pu. 2005 Sep;23(5):487-91.
10
On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identification.用于毛细管电泳无鞘电喷雾电离质谱的柱上样品富集：肽分析和蛋白质鉴定的评估
Anal Chem. 2003 Nov 1;75(21):5984-93. doi: 10.1021/ac0301548.

本文引用的文献

1
Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds.集成单细胞提取和电裂解用于细胞内化合物质谱检测的微流控装置。
Electrophoresis. 2023 Jan;44(1-2):313-322. doi: 10.1002/elps.202100379. Epub 2022 Apr 18.
2
Current applications of capillary electrophoresis-mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid-2021): A review.毛细管电泳-质谱法在尿液中生物重要分析物分析中的当前应用（2017 年至 2021 年年中）：综述。
J Sep Sci. 2022 Jan;45(1):305-324. doi: 10.1002/jssc.202100621. Epub 2021 Oct 7.
3
Application of capillary electrophoresis-nano-electrospray ionization-mass spectrometry for the determination of N-nitrosodimethylamine in pharmaceuticals.
毛细管电泳-纳升电喷雾电离质谱法在药物中 N-亚硝基二甲胺测定中的应用。
Electrophoresis. 2021 Feb;42(4):334-341. doi: 10.1002/elps.202000303. Epub 2021 Jan 14.
4
Capillary zone electrophoresis-mass spectrometry for top-down proteomics.用于自上而下蛋白质组学的毛细管区带电泳-质谱联用技术
Trends Analyt Chem. 2019 Nov;120. doi: 10.1016/j.trac.2019.115644. Epub 2019 Aug 28.
5
Advances in capillary electrophoresis for the life sciences.毛细管电泳在生命科学中的进展。
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jun 15;1118-1119:116-136. doi: 10.1016/j.jchromb.2019.04.020. Epub 2019 Apr 12.
6
Microfabricated liquid junction hybrid capillary electrophoresis-mass spectrometry interface for fully automated operation.微制造的液体结混合毛细管电泳-质谱接口，用于全自动操作。
Electrophoresis. 2019 Sep;40(18-19):2263-2270. doi: 10.1002/elps.201900049. Epub 2019 Feb 28.
7
Refolding kinetics of cytochrome c studied with microsecond timescale continuous-flow UV-vis spectroscopy and rapid freeze-quench EPR.用微秒时间尺度连续流动紫外可见光谱和快速冷冻淬灭 EPR 研究细胞色素 c 的重折叠动力学。
J Inorg Biochem. 2018 Jul;184:42-49. doi: 10.1016/j.jinorgbio.2018.04.011. Epub 2018 Apr 11.
8
Cytochrome c as a Potentially Clinical Useful Marker of Mitochondrial and Cellular Damage.细胞色素c作为线粒体和细胞损伤潜在的临床有用标志物
Front Immunol. 2016 Jul 20;7:279. doi: 10.3389/fimmu.2016.00279. eCollection 2016.
9
Ionization and transmission efficiency in an electrospray ionization-mass spectrometry interface.电喷雾电离-质谱联用接口中的电离与传输效率
J Am Soc Mass Spectrom. 2007 Sep;18(9):1582-90. doi: 10.1016/j.jasms.2007.05.018. Epub 2007 Jun 2.
10
A fully integrated monolithic microchip electrospray device for mass spectrometry.一种用于质谱分析的完全集成的单片微芯片电喷雾装置。
Anal Chem. 2000 Sep 1;72(17):4058-63. doi: 10.1021/ac000325y.