University of Ngaoundere, Faculty of Sciences, Department of Biological Sciences, P. O. Box 454, Ngaoundere, Cameroon.
University of Veterinay Medicine Hannover, Institute for Parasitology, Centre for Infection Medicine, Buenteweg 17, Hannover D-30559, Germany.
Vet Parasitol. 2024 Oct;331:110288. doi: 10.1016/j.vetpar.2024.110288. Epub 2024 Aug 15.
Parasitic infections with gastrointestinal nematodes are a serious problem for the health and welfare of domestic animals and negatively affect the economics of animal production. Haemonchus contortus is a haematophagous nematode of small ruminants responsible for significant mortality and morbidity. In addition, the widespread resistance to synthetic anthelmintic drugs emphasizes the urgent need of alternative treatment options against haemonchosis. This work aims to investigate the anthelmintic activity of an hydroethanolic Combretum mucronatum leaf extract (CMLE) against Haemonchus contortus in goats. Goats were artificially infected with 3500 third-stage larvae of H. contortus, and 21 days later, treated with CMLE (1000, 500, 250 mg/kg) for 4 consecutive days. Different parameters such as faecal egg count reduction, weight and haematocrit were monitored during the experimental period. The number of eggs per gram of faeces (EPG) was concentration-depended lower and significantly reduced compared to the untreated control (p < 0.0001). The effect of the highest CMLE dose (4 ×1000 mg/kg body weight) was similar to the effect of albendazole (1 ×5 mg/kg of body weight). The ED and ED values calculated were 189.17 and 392.33 mg/kg body weight respectively. ED and ED values were time-dependent. Moreover, CMLE improved haematocrit and weight of goats in dose-dependent and time-dependent manner. These results showed that CMLE could be used for haemonchosis treatment in small ruminants.
胃肠道线虫寄生虫感染是家畜健康和福利的严重问题，并对动物生产的经济效益产生负面影响。捻转血矛线虫是反刍动物的一种血吸性线虫，可导致重大的死亡率和发病率。此外，广泛存在的对合成驱虫药物的耐药性强调了迫切需要替代的抗捻转血矛线虫病治疗方案。本工作旨在研究 Combretum mucronatum 叶的水醇提取物（CMLE）对山羊捻转血矛线虫的驱虫活性。山羊被人工感染 3500 条捻转血矛线虫的第三期幼虫，21 天后，用 CMLE（1000、500、250mg/kg）连续 4 天治疗。在实验期间监测粪便虫卵减少率、体重和红细胞压积等不同参数。粪便中每克虫卵数（EPG）呈浓度依赖性降低，与未处理对照组相比显著降低（p<0.0001）。最高 CMLE 剂量（4×1000mg/kg 体重）的效果与阿苯达唑（1×5mg/kg 体重）的效果相似。计算得出的 ED 和 ED 值分别为 189.17 和 392.33mg/kg 体重。ED 和 ED 值是时间依赖性的。此外，CMLE 以剂量和时间依赖性方式改善了山羊的红细胞压积和体重。这些结果表明，CMLE 可用于治疗反刍动物的捻转血矛线虫病。
