Key Laboratory of Textile Science & Technology of Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; Textile engineering department, Bahir Dar University, Bahir Dar 6000, Ethiopia.
Key Laboratory of Textile Science & Technology of Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China.
Int J Biol Macromol. 2024 Nov;279(Pt 2):134934. doi: 10.1016/j.ijbiomac.2024.134934. Epub 2024 Aug 22.
Rhamnus prinoides leaf contains carbohydrates, saccharides, phenolic acids, and diterpenes with antibacterial, wound-healing, and anti-inflammatory properties. In this study, Rhamnus prinoides leaf extract was successfully incorporated into polycaprolactone-cellulose acetate (PCL-CA) nanofibers through electrospinning technique for the first time. The mats' morphology, diameter, chemical, and crystalline structure were characterized. The study investigated the mats' antibacterial activity, wound healing, cytotoxicity, drug release behaviour, hydrophilicity, and water absorbency properties. The results revealed that the mats exhibited continuous, smooth, without-beads, and interconnected structures, with average fiber diameters ranging from 385 ± 21 nm to 332 ± 74 nm. The antibacterial effeciency was remarkable against S. aureus and E. coli, achieving bacterial reduction percentages exceeding 99 % at concentrations of 3 % and above against S. aureus and 5 % and above against E. coli. Cytotoxic tests showed low-cytotoxicity up to an extract concentration of 7 %. The extract release increases with an increase in concentration. In vitro wound healing assay, the mats enhanced cell migration to the wound area. Additionally, the incorporation of Rhamnus prinoides significantly improved the hydrophilicity and water absorbency of the nanofibers. Overall, the study highlights the mats' broad antimicrobial and wound healing properties with less cytotoxicity, hydrophilicity, and water absorbency, making them promising for use as wound dressings.
鼠李叶含有碳水化合物、糖、酚酸和二萜，具有抗菌、愈合伤口和抗炎的特性。在这项研究中，鼠李叶提取物首次通过静电纺丝技术成功地掺入到聚己内酯-醋酸纤维素（PCL-CA）纳米纤维中。对垫子的形态、直径、化学和晶体结构进行了表征。研究了垫子的抗菌活性、伤口愈合、细胞毒性、药物释放行为、亲水性和吸水性。结果表明，垫子呈现连续、光滑、无珠、相互连接的结构，平均纤维直径从 385 ± 21nm 到 332 ± 74nm 不等。垫子对金黄色葡萄球菌和大肠杆菌具有显著的抗菌效率，在浓度为 3%及以上时对金黄色葡萄球菌的细菌减少率超过 99%，在浓度为 5%及以上时对大肠杆菌的细菌减少率超过 99%。细胞毒性测试表明，提取物的浓度高达 7%时仍具有低细胞毒性。提取物的释放量随浓度的增加而增加。体外伤口愈合试验表明，垫子促进了细胞向伤口区域的迁移。此外，鼠李叶的掺入显著提高了纳米纤维的亲水性和吸水性。总的来说，该研究强调了垫子具有广泛的抗菌和伤口愈合特性，同时具有较低的细胞毒性、亲水性和吸水性，有望用作伤口敷料。
