Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2019 Nov 1;140:1260-1268. doi: 10.1016/j.ijbiomac.2019.08.207. Epub 2019 Aug 28.
Cellulose acetate (CA) electrospun nanofibers are one of the most practical cellulosic material which normally applied as carriers for drug delivery and wound healing systems. In this study, CA and polycaprolactone (PCL) was applied to fabricate the electrospun nanofibrous for wound dressing application. Propolis is a resin-like macromolecule produced by honeybees from the buds and diverse plants. Among many applications of this macromolecule, it has been occasionally employed directly to the skin for wound healing applications. Herein, owing to the significance of propolis, CA/PCL nanofibers were impregnated with a propolis-extracted solution to reach antibacterial and antioxidant mat. The scanning electron microscopy (SEM) images revealed that electrospinning of 10% (w/w) CA along with 14% (w/w) PCL produced excellent nanofibers compared to the resultant nanofibers. Hydrophobicity/hydrophilicity nature of CA/PCL mats was measured using water contact-angle method before and after treatment with NaOH. The nanofibrous mats exhibited a high water absorption capacity of about 400%. Antioxidant effect was measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay and propolis-CA/PCL presented a high antioxidant activity. Additionally, propolis-CA/PCL mats showed antibacterial activity against both the Gram-positive and Gram-negative bacteria. In conclusion, our results have confirmed that the propolis-impregnated CA/PCL mats have provided an appropriate surface for wound healing system.
醋酸纤维素（CA）静电纺纳米纤维是最实用的纤维素材料之一，通常用作药物输送和伤口愈合系统的载体。在这项研究中，CA 和聚己内酯（PCL）被应用于制造用于伤口敷料应用的静电纺纳米纤维。蜂胶是蜜蜂从芽和各种植物中产生的树脂状大分子。在这种大分子的许多应用中，它偶尔会直接应用于皮肤，用于伤口愈合应用。在此，由于蜂胶的重要性，CA/PCL 纳米纤维被浸渍在蜂胶提取溶液中，以达到抗菌和抗氧化的效果。扫描电子显微镜（SEM）图像显示，与 10%（w/w）CA 共纺的 14%（w/w）PCL 产生的纳米纤维优于其他纳米纤维。CA/PCL 垫的疏水性/亲水性性质使用水接触角法在处理前后进行测量NaOH。纳米纤维垫的吸水率约为 400%。抗氧化效果通过 2,2-二苯基-1-苦基肼（DPPH）测定法进行测量，蜂胶-CA/PCL 表现出高抗氧化活性。此外，蜂胶-CA/PCL 垫对革兰氏阳性菌和革兰氏阴性菌均具有抗菌活性。总之，我们的结果证实，蜂胶浸渍的 CA/PCL 垫为伤口愈合系统提供了合适的表面。
