Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Nanotechnology, School of Advanced Medical Science and Technology, Shiraz University of Medical Sciences, Shiraz, Iran; Guilan Road Trauma Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Biol Macromol. 2024 Oct;278(Pt 3):134937. doi: 10.1016/j.ijbiomac.2024.134937. Epub 2024 Aug 22.
Trinitroglycerin (TNG) with remarkable angiogenic, antibacterial, and antioxidative activity is a promising candidate to govern wound healing capacity. However, its clinical administration is limited due to associated complications and NO short half-life. In the current study, TNG-loaded chitosan nanogels (TNG-Ngs) were examined in-vitro and in-vivo to gain insight into their clinical application. We prepared TNG-Ngs and characterized their physiochemical properties. The potential of TNG-Ngs was assessed using biocompatibility, scratch assay, and a full-thickness skin wounds model, followed by histopathological and immunohistochemistry examinations. TNG-Ngs particle size 96 ± 18 and definite size distribution histogram. The loading capacity (LC) and encapsulation efficiency (EE) of prepared TNG-Ngs were 70.2 % and 2.1 %, respectively. The TNG-Ngs samples showed enhanced migration of HUVECs with no apparent cytotoxicity. The topical use of TNG-Ngs on the wounds revealed a complete wound closure ratio, skin component formation, less scar width, remarkable granulation tissue, promoted collagen deposition, and enhanced the relative mean density of α-SMA and CD. TNG-Ngs accelerated wound healing by promoting collagen deposition and angiogenic activity, as well as reducing inflammation. The findings indicated that TNG-Ngs is expected to be well vascularized in the wound area and to be more effective in topical therapy.
三硝酸甘油酯（TNG）具有显著的血管生成、抗菌和抗氧化活性，是一种很有前途的治疗伤口愈合能力的候选药物。然而，由于其相关的并发症和 NO 半衰期短，其临床应用受到限制。在本研究中，我们研究了负载 TNG 的壳聚糖纳米凝胶（TNG-Ngs）的体内和体内特性，以了解其临床应用。我们制备了 TNG-Ngs 并对其理化性质进行了表征。通过细胞相容性试验、划痕试验和全层皮肤伤口模型评估了 TNG-Ngs 的潜力，随后进行了组织病理学和免疫组织化学检查。TNG-Ngs 的粒径为 96±18nm，具有明确的粒径分布直方图。制备的 TNG-Ngs 的载药量（LC）和包封率（EE）分别为 70.2%和 2.1%。TNG-Ngs 样品显示出增强的 HUVEC 迁移能力，且没有明显的细胞毒性。TNG-Ngs 局部应用于伤口后，完全闭合比例高，皮肤成分形成，疤痕宽度较小，肉芽组织明显，促进胶原蛋白沉积，并增加α-SMA 和 CD 的相对平均密度。TNG-Ngs 通过促进胶原蛋白沉积和血管生成活性以及减少炎症来加速伤口愈合。研究结果表明，TNG-Ngs 有望在伤口区域得到良好的血管化，并在局部治疗中更有效。
