Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran; Immunoregulation Research Center, Shahed University, Tehran, Iran.
Int J Biol Macromol. 2024 Oct;278(Pt 4):134980. doi: 10.1016/j.ijbiomac.2024.134980. Epub 2024 Aug 22.
The design and development of wound-dressing hydrogels with desirable therapeutic effects and proper mechanical and self-healing properties are crucial in the healthcare sector. This research aims to prepare a new self-healing hydrogel based on Tragacanth, polyvinyl alcohol, and borax to be used as a wound dressing, the hydrogel was first prepared through a simple and one-pot reaction. The efficiency of the resulting product was then assessed based on the rheological and self-healing tests as well as cellular tests on a mouse fibroblast cell line (L929) including toxicity and scratch tests as well as the investigation of the expression of TGFβ1, TGFβ2, and VEGF-A gens (using Real-time PCR). The synthesized hydrogel exhibited proper mechanical strength, high self-healing features, and no toxicity (cell viability >100 %). Rheological studies indicate that hydrogels with a higher borax content (PVA: B ratio of 5:1) exhibit a higher storage modulus across all frequencies. The presence of hydrogel improved the migration of the L929 cells and scratch healing. The hydrogel also caused a significant improvement in the expression of the growth factors of the genes (P < 0.001). Therefore, it can be concluded that the prepared wound dressing can actively contribute to wound healing, opening promising potentials in medical applications.
在医疗保健领域，设计和开发具有理想治疗效果、适当机械性能和自修复性能的伤口敷料水凝胶至关重要。本研究旨在制备一种基于刺云实胶、聚乙烯醇和硼砂的新型自修复水凝胶，用作伤口敷料。该水凝胶首先通过简单的一锅反应制备。然后，根据流变学和自修复测试以及对小鼠成纤维细胞系（L929）的细胞测试（包括毒性和划痕测试以及对 TGFβ1、TGFβ2 和 VEGF-A 基因表达的研究（使用实时 PCR））来评估所得产物的效率。合成的水凝胶表现出适当的机械强度、高自修复特性和无毒性（细胞活力>100%）。流变学研究表明，硼砂含量较高的水凝胶（PVA:B 比为 5:1）在所有频率下均表现出较高的储能模量。水凝胶的存在促进了 L929 细胞的迁移和划痕愈合。水凝胶还显著改善了基因生长因子的表达（P<0.001）。因此，可以得出结论，所制备的伤口敷料可以积极促进伤口愈合，为医学应用带来广阔的潜力。
