Center for Immunity and Inflammation, Department of Medicine, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, USA.
Wellcome Centre for Integrative Parasitology, School of Infection and Immunity, University of Glasgow, Glasgow, UK.
Life Sci Alliance. 2024 Aug 23;7(11). doi: 10.26508/lsa.202302249. Print 2024 Nov.
Skin wound healing due to full thickness wounds typically results in fibrosis and scarring, where parenchyma tissue is replaced with connective tissue. A major advance in wound healing research would be to instead promote tissue regeneration. Helminth parasites express excretory/secretory (ES) molecules, which can modulate mammalian host responses. One recently discovered ES protein, TGF-β mimic (TGM), binds the TGF-β receptor, though likely has other activities. Here, we demonstrate that topical administration of TGM under a Tegaderm bandage enhanced wound healing and tissue regeneration in an in vivo wound biopsy model. Increased restoration of normal tissue structure in the wound beds of TGM-treated mice was observed during mid- to late-stage wound healing. Both accelerated re-epithelialization and hair follicle regeneration were observed. Further analysis showed differential expansion of myeloid populations at different wound healing stages, suggesting recruitment and reprogramming of specific macrophage subsets. This study indicates a role for TGM as a potential therapeutic option for enhanced wound healing.
由于全层伤口，皮肤伤口愈合通常会导致纤维化和瘢痕形成，其中实质组织被结缔组织取代。伤口愈合研究的一项重大进展将是促进组织再生。寄生虫表达排泄/分泌（ES）分子，这些分子可以调节哺乳动物宿主的反应。最近发现的一种 ES 蛋白，TGF-β 模拟物（TGM），结合 TGF-β 受体，但可能具有其他活性。在这里，我们证明了在 Tegaderm 绷带下局部给予 TGM 可增强体内伤口活检模型中的伤口愈合和组织再生。在伤口愈合的中期到后期观察到 TGM 处理的小鼠的伤口床中正常组织结构的恢复增加。观察到加速再上皮化和毛囊再生。进一步分析表明，在不同的伤口愈合阶段，髓样细胞群体的差异扩张，表明特定巨噬细胞亚群的募集和重编程。这项研究表明 TGM 作为一种增强伤口愈合的潜在治疗选择的作用。
