热应激后性成熟小鼠睾丸发育的 RNA-seq 图谱。
The RNA-seq mapping of Testicular Development after Heat Stress in Sexually Mature Mice.
机构信息
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
出版信息
Sci Data. 2024 Aug 23;11(1):913. doi: 10.1038/s41597-024-03782-3.
The testis serves as the primary site for spermatogenesis in mammals and is a crucial organ for the secretion of male hormones. Heat stress (HS) can have adverse effects on the seminiferous tubules, sperm quality, and sperm fertilization capability within the testis. Despite numerous previous studies describing various time points after heat stress in mice, a systematic and comprehensive dataset on heat stress and recovery in mice has been lacking. This study aimed to explore the gene expression changes in the recovery of multiple seminiferous epithelial cycles and spermatogenic cycles in mouse testicles after heat stress. We obtained high-throughput bulk RNA-seq data from testicular tissue of 4 NC mice and 32 HS mice (divided into 9 groups: NC, 30 min, 2 h, 6 h, 24 h, 3d, 8d, 24d, 47d, and 95d) and illustrated the dynamic changes in differential genes. This data set provides valuable insights into the detailed dynamic changes of one or more spermatogenic cycles after heat stress in mouse testicles, as well as the molecular mechanisms involved.
睾丸是哺乳动物精子发生的主要场所，也是男性激素分泌的关键器官。热应激（HS）会对睾丸的曲细精管、精子质量和精子受精能力产生不良影响。尽管之前有许多研究描述了小鼠热应激后的不同时间点，但缺乏关于小鼠热应激和恢复的系统和全面的数据集。本研究旨在探讨热应激后小鼠睾丸多个生精上皮周期和精子发生周期恢复过程中的基因表达变化。我们从 4 只 NC 小鼠和 32 只 HS 小鼠的睾丸组织中获得了高通量 bulk RNA-seq 数据（分为 9 组：NC、30min、2h、6h、24h、3d、8d、24d、47d 和 95d），并说明了差异基因的动态变化。这个数据集提供了有价值的见解，揭示了小鼠睾丸热应激后一个或多个精子发生周期的详细动态变化，以及涉及的分子机制。
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