College of Life Sciences, Shihezi University, Shihezi, 832003, Xinjiang, China.
Institute of Microbiological Application, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, Xinjiang, China.
Curr Microbiol. 2024 Aug 24;81(10):324. doi: 10.1007/s00284-024-03837-z.
It turns out that the more than trillion microorganisms living in the host's digestive tract are crucial for maintaining nutrient intake, environmental suitability, and physiological mechanism. Xinjiang fine-wool sheep is an exclusive breed for wool in China, which has excellent stress tolerance. In this study, we collected feces and blood samples of 20 Xinjiang fine-wool sheep under the same genetic characteristics, the Fine-Wool Sheep (FWS) group and the Control Fine-Wool Sheep (CFWS) group were set up according to the differs in phenotypic characteristics of their wool. By 16S rRNA amplicon sequence, ITS1 region amplicons and Targeted Metabolomics, we analyzed the microbial community structure of fecal microorganisms and Short Chain Fatty Acids (SCFAs) in serum of the Xinjiang fine-wool sheep. Fecal microbial sequencing showed that the bacterial composition and structure were similar between the two groups, whereas there were significant differences in the composition and structure of the fungal community. It was also found that the abundant of Neocallimastigomycota in the intestinal fungal community of FWS was higher. In addition, the results of the serum SCFAs content analysis showed that butyric acid was significantly differences than those two groups. Correlation analysis between SCFAs and bacteria found that butyric acid metabolism had positively correlated (P < 0.05) with Ruminococcus and UCG-005. Overall, our data provide more supplement about the gut microbes community composition and structure of the Xinjiang fine-wool sheep. These results might be useful for improving gut health of sheep and taking nutritional control measure to improve production traits of animals in future.
事实证明，宿主消化道中生活的超过万亿的微生物对于维持营养摄入、环境适应性和生理机制至关重要。新疆细毛羊是中国特有的毛用绵羊品种，具有优异的应激耐受性。在这项研究中，我们收集了 20 只具有相同遗传特征的新疆细毛羊的粪便和血液样本，根据羊毛表型特征的不同，设立了细毛羊（FWS）组和对照细毛羊（CFWS）组。通过 16S rRNA 扩增子序列、ITS1 区扩增子和靶向代谢组学分析，我们分析了新疆细毛羊粪便微生物和血清中短链脂肪酸（SCFAs）的微生物群落结构。粪便微生物测序结果表明，两组细菌组成和结构相似，而真菌群落的组成和结构存在显著差异。还发现 FWS 肠道真菌群落中 Neocallimastigomycota 的丰度较高。此外，血清 SCFAs 含量分析结果表明，两组间丁酸含量存在显著差异。SCFAs 与细菌的相关性分析发现，丁酸代谢与 Ruminococcus 和 UCG-005 呈正相关（P < 0.05）。总体而言，我们的数据提供了更多关于新疆细毛羊肠道微生物群落组成和结构的补充信息。这些结果可能有助于改善绵羊的肠道健康，并在未来采取营养控制措施来改善动物的生产性状。
