Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, 266071, China.
Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao, China.
Sci Data. 2024 Aug 24;11(1):919. doi: 10.1038/s41597-024-03700-7.
The Pleurotomarioidea, commonly referred to as slit shells, constitute one of the most ancient and enduring lineages within the phylum Mollusca, with its fossil record tracing back to the Upper Cambrian epoch. Its rareness and evolutionary antiquity surpass even that of the nautilus. In this study, we conducted the first transcriptome sequencing and analyses of Entemnotrochus rumphii (Schepman, 1879), a representative species of Pleurotomarioidea. Full-length transcriptome sequencing of E. rumphii was performed using the PacBio Sequel II platform with SMRT technology. A total of 64.38 gigabytes of data and 964,550 polymerase reads were generated, resulting in 28,068,998 subreads after data filtering. After de-duplication, correction, and clustering, we identified 19,273 genes. Additionally, next-generation sequencing was performed on 11 tissues of E. rumphii. This investigation provides a detailed portrayal and analytical scrutiny of its transcriptomic landscape.
海扇蛤目，通常被称为缝栖蛤，是软体动物门中最古老和持久的谱系之一，其化石记录可追溯到上寒武纪。它的稀有性和进化古老性甚至超过了鹦鹉螺。在这项研究中，我们对 Pleurotomarioidea 的代表物种 Entemnotrochus rumphii（Schepman，1879）进行了首次转录组测序和分析。使用 PacBio Sequel II 平台和 SMRT 技术对 E. rumphii 进行全长转录组测序。共产生了 64.38 千兆字节的数据和 964,550 个聚合酶读取，经过数据过滤后得到 28,068,998 个子读取。经过去重、校正和聚类，我们鉴定出 19,273 个基因。此外，还对 E. rumphii 的 11 种组织进行了下一代测序。这项研究提供了对其转录组图谱的详细描述和分析。
